南方医科大学学报2025，Vol.45Issue(6)：1143-1152,10.DOI:10.12122/j.issn.1673-4254.2025.06.04
扶正化积汤治疗非小细胞肺癌的分子机制:基于网络药理学及体外实验验证
Inhibitory effect of Fuzheng Huaji Decoction against non-small cell lung cancer cells in vitro and the possible molecular mechanism
摘要
Abstract
Objective To investigate the inhibitory effect of Fuzheng Huaji Decoction against non-small cell lung cancer(NSCLC)cells in vitro and explore the underlying mechanism.Methods The active ingredients and targets of Fuzheng Huaji Decoction were identified using TCMSP and SwissTargetPrediction databases.NSCLC-related targets from GeneCards and PharmGKB were intersected with the targets of the Decoction,and a protein-protein interaction(PPI)network was constructed to identify the core targets,which were analyzed with GO and KEGG pathway enrichment analysis.Cultured A549 cells were treated with different concentrations of Fuzheng Huaji Decoction-medicated serum,and the changes in cell proliferation,apoptosis,and protein expressions were examined using CCK-8 assay,annexin V-FITC/PI staining and Western blotting.Results Fuzheng Huaji Decoction contained 140 active ingredients,and 707 drug-disease intersecting targets were identified.Among these targets,TP53,AKT1,HIF1A,GAPDH,ALB,EGFR,CTNNB1,and TNF were identified as the core targets which were involved in the biological processes related to kinases and receptors and the PI3K-AKT,Ras,calcium,and MAPK pathways.Molecular docking studies indicated strong binding affinity of the active ingredients with TP53,AKT1,and HIF1A.In cultured A549 cells,treatment with 2.5%,5%,and 10%Fuzheng Huaji Decoction-medicated serum significantly inhibited cell proliferation,promoted cell apoptosis,and downregulated the expression levels of HIF1A,p-AKT(Thr308),and TP53 proteins.Conclusion Fuzheng Huaji Decoction inhibits proliferation of NSCLC cells possibly by downregulating the expressions of HIF1A,p-AKT(Thr308),and TP53.关键词
扶正化积汤/非小细胞肺癌/网络药理学/分子对接/体外实验Key words
Fuzheng Huaji Decoction/non-small cell lung cancer/network pharmacology/molecular docking/in vitro experiments引用本文复制引用
何丽君,陈晓菲,闫陈昕,师林..扶正化积汤治疗非小细胞肺癌的分子机制:基于网络药理学及体外实验验证[J].南方医科大学学报,2025,45(6):1143-1152,10.基金项目
国家自然科学基金(82274249) Supported by National Natural Science Foundation of China(82274249). （82274249）
