广西医科大学学报2025，Vol.42Issue(3)：360-367,8.DOI:10.16190/j.cnki.45-1211/r.2025.03.005
运动干预对2型糖尿病动脉粥样硬化小鼠内皮祖细胞功能的作用
The effects of exercise intervention on the function of endothelial progenitor cells in athero-sclerotic mice with type 2 diabetes mellitus
摘要
Abstract
Objective:To investigate the effects of different exercise modalities on body weight,blood glucose,and atherosclerotic plaques in mice with type 2 diabetes mellitus(T2DM)and atherosclerosis,and further com-pare the impacts of these exercise modalities on the function of endothelial progenitor cells(EPCs).Methods:ApoE-/-mice induced by high-fat feeding combined with streptozotocin(STZ)were constructed into T2DM ath-erosclerosis model,and 32 mice were randomly divided into blank control group(control group),aerobic exercise(AE)group,resistance exercise(RE)group and aerobic combined with resistance exercise(AE+RE)group.Mice were assessed for the changes of body weight and fasting blood glucose at 0,2,4,6,and 8 weeks of exercise in-tervention.After 8 weeks,the mouse aortas were isolated for Oil Red O staining and hematoxylin-eosin(HE)staining,and the mouse bone marrow EPCs were extracted from each group for proliferation,migration,adhe-sion,and angiogenesis assessments.Results:After 8 weeks of exercise intervention,there were significant differ-ences in the weight loss between the AE group and the AE+RE group compared with the control group(P＜0.05).Blood glucose levels were significantly decreased in all exercise groups(P＜0.05).Oil Red O staining of the aorta showed a significant reduction in the ratio of atherosclerotic plaques to the total vessel area in all exercise groups compared with the control group(P＜0.05).HE staining of aortic sections results indicated no significant difference in plaque-to-vessel diameter ratio between the AE and RE groups compared with the control group(P＞0.05),while the ratio in the AE+RE group was smaller(P＜0.05).EPCs in all exercise groups showed signifi-cant improvements in proliferation,migration and angiogenesis compared with the control group(P＜0.05).Ad-hesion ability was not significantly different between AE and RE groups compared with the control group(P＞0.05),while the AE+RE group showed significantly better adhesion(P＜0.05),and the difference was statisti-cally significant.Conclusion:Compared with single AE or RE methods,AE combined with RE exercise has a better improvement effect on body weight,blood glucose and atherosclerosis in T2DM atherosclerosis mice.It also has a significant positive effect on the promotion of EPCs function.关键词
2型糖尿病/动脉粥样硬化/运动/内皮祖细胞Key words
type 2 diabetes mellitus/atherosclerosis/exercise/endothelial progenitor cells分类
临床医学引用本文复制引用
黄峥,徐子岚,李苏蓝,吴妮晓,戴霞..运动干预对2型糖尿病动脉粥样硬化小鼠内皮祖细胞功能的作用[J].广西医科大学学报,2025,42(3):360-367,8.基金项目
国家自然科学基金资助项目(No.82160440) （No.82160440）
广西自然科学基金资助项目(No.2023GXNSFAA026323) （No.2023GXNSFAA026323）
广西研究生教育创新计划资助项目(No.YCSW2024246) （No.YCSW2024246）
