广西医科大学学报2025，Vol.42Issue(3)：368-376,9.DOI:10.16190/j.cnki.45-1211/r.2025.03.006
FeOx/NC纳米酶清除ROS保护软骨细胞的作用研究
The protective effects of FeOx/NC nanozymes on chondrocytes by scavenging reactive oxygen species
摘要
Abstract
Objective:To investigate the chondroprotective effects and underlying mechanisms of iron oxide-based nanozymes(FeOx/NC)in a rat model of osteoarthritis(OA).Methods:FeOx/NC nanozymes were synthe-sized and characterized,and their enzyme-mimetic activities resembling superoxide dismutase(SOD),catalase(CAT),and glutathione peroxidase(GPx)were detected.Primary chondrocytes were isolated in vitro from SD rats and divided into four groups:control group,H2O2 group,NC+H2O2 group,and FeOx/NC+H2O2 group.The biocompatibility was assessed using the cell counting kit-8(CCK-8)assay and Calcein-AM/PI staining,and reac-tive oxygen species(ROS)-scavenging ability was examined with ROS probes.Gene and protein expression lev-els of MMP-13 and IL-6 were measured via reverse transcription-quantitative polymerase chain reaction(RT-qPCR),immunofluorescence,and western blotting.Twenty rats were randomly assigned to four groups:sham group,saline group,NC group,and FeOx/NC group.Except for the sham group,OA models were established by anterior cruciate ligament transection.Joint morphology was observed,and histopathological changes were evalu-ated by hematoxylin-eosin(HE)and Safranin O-Fast Green staining.Results:TEM analysis confirmed the suc-cessful synthesis of FeOx/NC,which exhibited SOD-,CAT-,and GPx-like activities.CCK-8 and Calcein-AM/PI assays demonstrated that FeOx/NC exhibited no significant cytotoxicity towards chondrocytes.The ROS probe analysis revealed significantly reduced fluorescence intensity in the FeOx/NC+H2O2 group compared with the H2O2 group(P＜0.05).RT-qPCR results showed that the mRNA expression levels of MMP-13 and IL-6 in the FeOx/NC+H2O2 group were significantly lower than those in the H2O2 group(P＜0.05).The results of immuno-fluorescence showed the fluorescence intensity of MMP-13 in the FeOx/NC+H2O2 group was significantly lower than that in the H2O2 group(P＜0.05).Western blotting demonstrated that the protein expression levels of MMP-13 and IL-6 were significantly decreased in the FeOx/NC+H2O2 group compared with the H2O2 group(P＜0.05).Macroscopic and histological evaluations demonstrated that FeOx/NC treatment alleviated cartilage damage com-pared with the saline group.Conclusion:FeOx/NC nanozymes exhibit excellent biocompatibility and possess multi-enzyme catalytic activity,enabling them to efficiently scavenge intracellular ROS in chondrocytes,thereby suppressing oxidative stress and alleviating the progression of OA.关键词
纳米酶/活性氧/骨关节炎Key words
nanozymes/reactive oxygen species/osteoarthritis分类
基础医学引用本文复制引用
郭剑锋,张荣炜,黄章睿,庞志权,罗世兴..FeOx/NC纳米酶清除ROS保护软骨细胞的作用研究[J].广西医科大学学报,2025,42(3):368-376,9.基金项目
国家自然科学基金资助项目(No.82160429) （No.82160429）
