广西医科大学学报2025，Vol.42Issue(3)：377-384,8.DOI:10.16190/j.cnki.45-1211/r.2025.03.007
基于类酶活性DNA-Ag/Pt双金属纳米簇的比色法检测血清中尿酸
Colorimetric assay of uric acid in serum based on DNA-Ag/Pt bimetallic nanoclusters with enzyme-like activity
摘要
Abstract
Objective:To establish a novel colorimetric sensor for uric acid in serum via DNA-Ag/Pt bimetallic nanoclusters(DNA-Ag/Pt NCs)with enzyme-like activity.Methods:Firstly,DNA-Ag/Pt NCs with high peroxidase-like activity were synthesized using single-stranded DNA as the template.The DNA-Ag/Pt NCs were characterized by transmission electron microscopy,dynamic light scattering,Zeta potential,X-ray photoelectron spectroscopy,X-ray diffractometer,electron resonance spectroscopy and Fourier transform infrared spectroscopy.Then,DNA-Ag/Pt NCs catalyzed the color reaction between H2O2 and 3,3',5,5'-tetramethylbenzidine,in which H2O2 was generated by the reaction of uricase and UA.The concentration of uric acid was quantitatively deter-mined based on the color changes and the measurement results by UV-vis spectrometer.Furthermore,this method was applied to the detection of uric acid concentration and the standard addition recovery experiments in human serum samples.Results:DNA-Ag/Pt NCs with high peroxidase-like activity were successfully prepared.This method showed a linear detection range for uric acid from 0.3 to 75 μmol/L,with a detection limit of 0.15 μmol/L.The concentration of uric acid in selected healthy human serum samples were between 203.04 μmol/L and 381.40 μmol/L,while that of samples from patients with hyperuricemia ranged from 420.97 μmol/L to 463.27 μmol/L.The standard addition recovery rate was in the range from 95.0%to 109.8%.Conclusion:The colorimet-ric assay based on DNA-Ag/Pt NCs for uric acid exhibits high specificity and sensitivity,and successfully achieves the detection of uric acid in serum samples.关键词
尿酸/尿酸酶/比色法/DNA-Ag/Pt双金属纳米簇/类过氧化物酶活性Key words
uric acid/uricase/colorimetric assay/DNA-Ag/Pt bimetallic nanoclusters/peroxidase-like activity引用本文复制引用
洪婉蓉,潘向和,覃英凤,刘金文..基于类酶活性DNA-Ag/Pt双金属纳米簇的比色法检测血清中尿酸[J].广西医科大学学报,2025,42(3):377-384,8.基金项目
国家自然科学基金资助项目(No.22266007) （No.22266007）
广西自然科学基金资助项目(No.2024GXNSFAA010208) （No.2024GXNSFAA010208）
长寿与老年相关疾病教育部重点实验室资助项目(No.KLLAD202302) （No.KLLAD202302）
