广西医科大学学报2025，Vol.42Issue(3)：385-394,10.DOI:10.16190/j.cnki.45-1211/r.2025.03.008
糖酵解关键基因ME1和ALDH3A1调控乳酸通路介导COPD相关免疫失调
The key genes ME1 and ALDH3A1 involved in glycolysis regulate the lactate pathway and me-diate COPD related immune dysregulation
摘要
Abstract
Objective:To investigate the expression of glycolysis-related genes in the pathogenesis of chronic ob-structive pulmonary disease(COPD)utilizing bioinformatics methods and the potential role of these genes in me-diating immune dysregulation in COPD through their influence on the glycolysis-controlled lactylation pathway.Methods:The COPD-related dataset GSE20257 was sourced from the GEO database,followed by differential gene correlation analysis and weighted gene co-expression network analysis(WGCNA).The relevant genes iden-tified from both datasets were intersected with the glycolysis gene set to filter for common differentially ex-pressed genes,which were subsequently subjected to enrichment analysis.Subsequently,key genes involved in glycolysis were identified using the LASSO and SVM-RFE algorithms.After subtyping,GSEA analysis was con-ducted.An immune infiltration analysis was performed to evaluate the extent of immune cell infiltration in COPD samples and explore the correlation between key genes and immune cells.Finally,the relative expression levels of glycolytic key genes in human and mouse lung tissues were assessed using reverse transcription-quantitative polymerase chain reaction(RT-qPCR).Results:After screening 16 commonly differentially expressed genes through WGCNA analysis and dataset intersection,ME1 and ALDH3A1 were ultimately identified as key genes using LASSO and SVM-RFE algorithms.Cluster analysis classified the samples into two subtypes,and GSEA analysis of the differentially expressed genes between these subtypes revealed significant enrichment in the cell cycle,chemokine signaling pathway,JAK-STAT signaling pathway,among others.Compared with the control group,the gene expression levels of ME1 and ALDH3A1 in the lung tissue of the COPD group were significantly increased(P＜0.05).Additionally,the gene expression levels of ME1 and ALDH3A1 in the lung tissue of mice in the chronic tobacco smoke exposure(CS)group were also significantly increased compared with those in the air group(P＜0.01).Conclusion:The glycolysis-related genes ME1 and ALDH3A1 may mediate immune dysregula-tion in COPD by regulating the lactate pathway through glycolysis.关键词
慢性阻塞性肺疾病/糖酵解相关基因/乳酸通路Key words
chronic obstructive pulmonary disease/glycolysis-related genes/lactate pathway分类
临床医学引用本文复制引用
谢文丽,周广,靖志涛,邱诗林..糖酵解关键基因ME1和ALDH3A1调控乳酸通路介导COPD相关免疫失调[J].广西医科大学学报,2025,42(3):385-394,10.基金项目
国家自然科学基金(青年科学基金项目)资助项目(No.81800037) （青年科学基金项目）
