广西医科大学学报2025，Vol.42Issue(3)：395-401,7.DOI:10.16190/j.cnki.45-1211/r.2025.03.009
Pd/ZIF-8纳米酶介导光热—酶促协同抗菌的研究
The synergistic antibacterial effects of photothermal and enzymatic catalysis mediated by Pd/ZIF-8 nanozyme
摘要
Abstract
Objective:To explore the synergistic antibacterial performance of photothermal and enzymatic ca-talysis mediated by Pd/ZIF-8 nanozyme,and assess its potential for biomedical applications.Methods:The Pd/ZIF-8 nanozyme was prepared by the hydrothermal method,and its structure was characterized by transmission electron microscopy(TEM)and X-ray diffraction(XRD).The photothermal conversion performance of the Pd/ZIF-8 nanozyme and its ability to mimic the activities of peroxidase(POD)and glutathione peroxidase(GPx)were evaluated.The cell counting kit-8(CCK-8)method was used to detect the toxicity of Pd/ZIF-8 to human der-mal fibroblasts(HDF-a),and the erythrocyte hemolysis test was used to detect its blood compatibility.The anti-bacterial performance of Pd/ZIF-8 against Gram-negative bacteria(Escherichia coli,E.coli)and Gram-positive bacteria(methicillin-resistant Staphylococcus aureus,MRSA)under the irradiation of 808 nm near-infrared light was detected.Results:TEM and XRD analyses confirmed the successful synthesis of the Pd/ZIF-8 nanozyme.The Pd/ZIF-8 nanozyme exhibited good photothermal performance with a photothermal conversion rate of 65.3%and good stability.Meanwhile,the Pd/ZIF-8 nanozyme exhibited excellent dual enzyme-like activities(POD and GPx).The results of cytotoxicity and hemolysis tests indicated that Pd/ZIF-8 had good biocompatibility.Under near infrared light irradiation,the antibacterial rates of Pd/ZIF-8 against E.coli and MRSA were 97.3%and 99.41%,respectively(P＜0.0001).Conclusion:The Pd/ZIF-8 nanozyme has the ability to mediate photothermal and enzymatic promotion to achieve synergistic antimicrobial properties.This nanomaterial shows promising ap-plication prospects in the field of anti-infection treatment and provides important ideas for the development of new antibacterial agents.关键词
纳米酶/近红外光/抗菌活性/酶催化Key words
nanozyme/near-infrared light/antibacterial activity/enzymatic catalysis分类
中医学引用本文复制引用
莫苗,黄章睿,赵昕,钟静萍..Pd/ZIF-8纳米酶介导光热—酶促协同抗菌的研究[J].广西医科大学学报,2025,42(3):395-401,7.基金项目
国家自然科学基金资助项目(No.52301303) （No.52301303）
