广西医科大学学报2025，Vol.42Issue(3)：402-407,6.DOI:10.16190/j.cnki.45-1211/r.2025.03.010
实时荧光定量PCR检测皮肤脓液标本在HIV阴性马尔尼菲篮状菌病诊断中的价值
Diagnostic value of reverse transcription-quantitative PCR in skin pus specimens for HIV-negative talaromycosis marneffei
摘要
Abstract
Objective:To evaluate the diagnostic value of reverse transcription-quantitative polymerase chain re-action(RT-qPCR)detection of skin pus specimens in HIV-negative patients with talaromycosis marneffei(TSM).Methods:Twenty-seven patients who had suspected TSM(22 confirmed TSM and 5 non-TSM,all HIV-negative)with skin pus were selected from the First Affiliated Hospital of Guangxi Medical University from January 2020 to December 2024.Meanwhile,skin pus and serum specimens of these 27 patients were collected for RT-qPCR,fungal culture,fungal microscopy,and serological tests,respectively.Results:In the specimens of 22 TSM-diagnosed patients,the positive rate of serum detected by RT-qPCR was 9.09%(2/22),while the positive rate of skin pus specimens detected by RT-qPCR reached 68.18%(15/22).There was a statistically significant difference in the positive rates between the two(P＜0.001).In addition,5 skin pus specimens from patients with confirmed TSM showed negative fungal culture results,but the same skin pus specimens were tested positive by RT-qPCR.The RT-qPCR results of serum and skin pus specimens from 5 non-TSM patients were negative.The results of this study suggested that the diagnostic efficacy of RT-qPCR detection using skin pus was higher than that of se-rum RT-qPCR,pus culture,pus microscopy,and serum galactomannan detection.Conclusion:RT-qPCR detec-tion using skin pus as a specimen can rapidly diagnose TSM infection and has a high application value in the early diagnosis of HIV-negative TSM patients.关键词
马尔尼菲篮状菌病/实时荧光定量聚合酶链式反应/人类免疫缺陷病毒阴性/皮肤脓液/诊断价值Key words
talaromycosis marneffei/reverse transcription-quantitative polymerase chain reaction/HIV-negative/skin pus/diagnostic value分类
临床医学引用本文复制引用
黄澜,黄啟华,郑冬燕,吴维轩,潘开素,郑艳青,廖万清,曹存巍..实时荧光定量PCR检测皮肤脓液标本在HIV阴性马尔尼菲篮状菌病诊断中的价值[J].广西医科大学学报,2025,42(3):402-407,6.基金项目
广西自然科学基金青年科学基金资助项目(No.2022GXNSFBA035509) （No.2022GXNSFBA035509）
广西研究生教育创新计划项目(No.YCBZ2024143) （No.YCBZ2024143）
广西重点研发计划项目资助(No.桂科AB24010134) （No.桂科AB24010134）
