中北大学学报（自然科学版）2025，Vol.46Issue(3)：359-368,10.DOI:10.62756/jnuc.issn.1673-3193.2024.09.0002
高效降解玉米秸秆细菌复合菌系的构建及降解效果分析
Construction of Bacterial Composite Strain with Efficient Degradation of Corn Straw and Analysis of Degradation Effect
摘要
Abstract
In order to solve the problem of difficult degradation and low efficiency of corn straw,this study used isolated and preserved cellulose degrading bacteria(Paenibacillus lautus)J1,(Paenibacillus chi-bensis)J2,(Paenibacillus vini)J3 and lignin degrading potential bacteria(Beijerinckia fluminensis)6-1H,(Pseudomonas extremaustralis)1-2D,(Bacillus subtilis)BS to construct highly efficient degrading bacteria complex bacteria D(J1∶J3∶6-1H∶BS=1∶1∶1∶1).The optimum enzyme production conditions and inter-nal degradation mechanism of complex bacterium D were studied.The results show that the optimal enzyme production conditions are:CMC-Na 10 g/L,peptone 10 g/L,pH 7,inoculation amount 9%and temperature 35℃.Under these conditions,the maximum enzyme activity of filter paper is 41.97 U/mL,which is 2.42 times of the maximum enzyme activity of single bacteria.After 7 days of treatment,the degradation rates of cellulose,hemicellulose and lignin are 59.1%,31.1%and 51.1%,respectively,and the degradation rate of straw reaches 46.2%,which is 1.95 times of the highest degradation rate of single bacteria.Scanning electron microscopy,Fourier transform infrared spectroscopy and X-ray diffraction analysis showes that the surface structure of corn straw is destroyed,the chemical bond strength of ligno-cellulose is weakened,and the crystallinity decreases from 57.32%to 55.77%.The results show that the constructed composite bacteria significantly enhances the degradation effect of lignocellulose and has good application potential.关键词
细菌复合菌系/滤纸酶活性/产酶条件优化/玉米秸秆降解Key words
bacterial complex strain/filter paper enzyme activity/optimization of enzyme production con-ditions/corn straw degradation分类
生物科学引用本文复制引用
韩群英,白红娟,宋雨,王子豪,卢静,贾亚婷..高效降解玉米秸秆细菌复合菌系的构建及降解效果分析[J].中北大学学报（自然科学版）,2025,46(3):359-368,10.基金项目
山西省留学回国人员科技活动择优资助项目(20240010) （20240010）
