中北大学学报（自然科学版）2025，Vol.46Issue(3)：369-378,10.DOI:10.62756/jnuc.issn.1673-3193.2024.08.0007
莠去津高效降解菌ADBD-13的降解机理及生物修复研究
Degradation Mechanism and Bioremediation of Atrazine Efficient Degrading Strain ADBD-13
摘要
Abstract
Atrazine is widely used to remove weeds in corn fields,but its residues have caused certain harm to human health and ecological environment.In this paper,5 strains of bacteria that could grow in medium with higher concentrations of atrazine and degrade atrazine were screened and isolated from the surface soil of corn fields where atrazine pesticide had been applied for a long time through enrichment and domestication.Morphological characteristics,16S rRNA sequence and ANI index were analyzed for the strains with the best degradation effect,and the species were identified.The degradation effect was deter-mined by high-performance liquid chromatography and the degradation mechanism was studied by HPLC-MS.The bioremediation effects on soil,wheat and maize were investigated by soil inoculation,pot experi-ment and hydroponic experiment.The results show that among the 5 strains,the one with the best degra-dation effect is ADBD-13.ADBD-13 is Paenarthrobacter ureafaciens,which can degrade 99.90%of atrazine at 100 mg/L after 70 hours of incubation.It has the best degradation effect at 30℃,pH 8.0,and the initial mass concentration of atrazine is 10 mg/L.It has certain acid and alkali resistance,high and low temperature resistance.The biodegradation product of ADBD-13 to atrazine is cyanuric acid after dechlorination,deisopropyl,hydroxylation,hydrodealkylation,elimination and hydrolysis.The applica-tion of strain ADBD-13 in the fermentation solution can degrade 70.54%of atrazine in the contaminated soil containing 50 mg/kg atrazine at 7 days.Under atrazine stress,the plant height,root length,fresh weight and dry weight of wheat seedlings and corn seedlings in the soil increases by at least 21%and 30%,respectively,compared with the control.And the four growth indexes of wheat seedlings and corn seedlings growing in water increases by at least 24%and 27%,respectively.These results indicate that Paenarthrobacter ureafaciens ADBD-13 has broad prospects in soil remediation and bioremediation.关键词
产脲节杆菌/莠去津/生物降解/生物修复/小麦/玉米Key words
Paenarthrobacter ureafaciens/atrazine/biodegradation/bioremediation/wheat/corn分类
环境科学引用本文复制引用
李蒙蒙,郭金春,刘珂良,何碧珀,刘红彦,吴仁海,刘颖,刘清浩..莠去津高效降解菌ADBD-13的降解机理及生物修复研究[J].中北大学学报（自然科学版）,2025,46(3):369-378,10.基金项目
财政部和农业农村部国家现代农业产业技术体系项目(CARS-14) （CARS-14）
河南省农科院优秀青年科技基金资助项目(2022YQ04) （2022YQ04）
河南省重点研发专项(221111112300) （221111112300）
