福建农林大学学报（自然科学版）2025，Vol.54Issue(4)：495-501,7.DOI:10.13323/j.cnki.j.fafu(nat.sci.).202409014
3株FAdV-Ⅰ在LMH细胞上的培养特性及不同病毒传代方法的比较
Cultivation characteristics of 3 FAdV-Ⅰ strains in LMH cells and comparison of different methods for virus passage
摘要
Abstract
[Objective]To provide a better passage method for fowl adenovirus group I(FAdV-Ⅰ),the culture characteristics of different FAdV-Ⅰ strains in leghorn male hepatoma(LMH)cells as well as merits and demerits of different virus passage methods were investigated.[Method]Three FAdV-Ⅰstrains(CELO,GY and TR22-CK8)were inoculated into LMH cells for virus passage and determination of passage number by observing its cytopathic effect.Then the virus was harvested by conventional method and freeze-thaw subculture,and the 50%tissue culture infective dose(TCID50)was determined.Different loads of FAdV-Ⅰwere added to chicken embryo allantoic fluids infected with infectious bronchitis virus(IBV),and the FAdV-Ⅰ load in the IBV live vaccine was detected by indirect immunofluorescence assay(IFA)after propagated by serial passage or freeze-thaw subculture.[Result]All the 3 FAdV-Ⅰ strains can induce the characteristic cytopathic effects of avian adenovirus on LMH cells,but with different timings and degrees.Passage with virus would worsen the cytopathic effects,but no more than 3 serial passages.The virus loads propagated by freeze-thaw subculture were higher,and the cells were less likely to detach.[Conclusion]Different FAdV-Ⅰstrains had various adaptability and culture characteristics on LMH cells,and freeze-thaw subculture was more suitable for the propagation of FAdV-Ⅰ.关键词
禽腺病毒Ⅰ群/鸡肝癌细胞/细胞培养特性/细胞病变/病毒传代Key words
fowl adenovirus groupⅠ/leghorn male hepatoma cells/cell culture characteristics/cytopathic effect/virus passage分类
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黄小洁,赵炜,刘丹,吴华伟,吴思谦,薛麒,廖晓光,陈政谕,孔冬妮,邓永,苏佳..3株FAdV-Ⅰ在LMH细胞上的培养特性及不同病毒传代方法的比较[J].福建农林大学学报（自然科学版）,2025,54(4):495-501,7.基金项目
广西高校中青年教师科研基础能力提升项目(2025KY1381) （2025KY1381）
兽药行业公益性重点专项(GY202401). （GY202401）
