福建农林大学学报（自然科学版）2025，Vol.54Issue(4)：502-512,11.DOI:10.13323/j.cnki.j.fafu(nat.sci.).202406036
半刺厚唇鱼源达卡气单胞菌的鉴定及其毒力基因与耐药性分析
Identification of Aeromonas dhakensis isolated from Acrossocheilius hemispinus and analysis on its virulence genes and antimicrobial resistance
摘要
Abstract
[Objective]The cause of rot skin disease on Acrossocheilius hemispinus was find out,to provide reference for the research and prevention of this disease.[Method]The pathogen of deseased Acrossocheilius hemispinus was isolated and identified,and then verifed by recurrent infection test.Antimicrobial resistance of the pathogen was analyzed by drug susceptibility test,and its virulence genes and drug resistance genes were identifed by PCR.[Result]Bacterial strain AhL210623NA,isolated from individual tissue of Acrossocheilius hemispinus with rot skin disease,was identified as Aeromonas dhakensis by morphological observation,physiological and biochemical identification,and sequence analysis of 16S rDNA and gyrB gene.Recurrent infection experiment showed the strain caused similar symptoms on healthy Acrossocheilius hemispinus,which verified the strain as the pathogen of rot skin disease.The le-thal dose(LD50)of the strain was 6.19×106 CFU·mL-1.And it carried 7 virulence genes of hlyA,aerA,alt,act,ahpA,epr and Hcp,but no ast,exu,lip nor ascV.Antibiotic sensitivity test showed that AhL210623NA was highly sensitive to aminoglycosides,polymyxins,tetracyclines,quinolones,chloramphenicols and cefotaxime of β-lactam,moderately sensitive to ceftazidime of β-lactams and erythromycin of macrolides,but resistant to penicillin,ampicillin,carboxpicillin and benzoxicillin of β-lactams,roxithromycin of macrolides,vancomycin of glycopeptides,cotrimoxazole of sulfanilamides.Resistence gene detection showed that the strain only carried sul1 gene,but not qnrA,qnrS,tetA,tetC,ant3,strA,tem and ctxM genes.[Conclusion]Aeromonas dhakensis was isolated from Acros-socheilius hemispinus for the first time.This bacterium has relatively strong toxicity,but has weak resistance to common antibiotics,in-dicating there are many treatment drugs available.关键词
半刺厚唇鱼/达卡气单胞菌/毒力基因/耐药基因/药物敏感性Key words
Acrossocheilius hemispinus/Aeromonas dhakensis/virulence gene/antibiotic resistance gene/antibiotic sensitivity分类
生物学引用本文复制引用
陈斌..半刺厚唇鱼源达卡气单胞菌的鉴定及其毒力基因与耐药性分析[J].福建农林大学学报（自然科学版）,2025,54(4):502-512,11.基金项目
福建省种业创新和产业化工程项目(2021FJSCZY04) （2021FJSCZY04）
福建省海洋服务与渔业高质量发展专项(FJHY-YYKJ-2022-2-7). （FJHY-YYKJ-2022-2-7）
