福建农林大学学报（自然科学版）2025，Vol.54Issue(4)：513-521,9.DOI:10.13323/j.cnki.j.fafu(nat.sci.).202409035
红嘴鸥IRF7基因克隆与生物信息学分析
Cloning and bioinformatics analysis of IRF7 gene in Larus ridibundus
摘要
Abstract
[Objective]This study focused on the relevant information of interferon regulatory factor 7(IRF7)gene in Larus ridibun-dus,to provide a basis for the functional study of protein IRF7 in antiviral response.[Method]The IRF7 gene was cloned,and its sequence was analyzed using MegAlign and MEGA 11.0 software.The biological characteristics of protein IRF7,including rare co-don characteristics,physicochemical properties,N-glycosylation sites,phosphorylation sites,transmembrane regions,signal pep-tides,affinity,hydrophobicity,subcellular localization,secondary structure and tertiary structure,were analyzed by software like Rare Codon Callor tool,ProtParam tool,NetNGlyc 1.0 tool,NetPhos 3.1 tool,TMHMM 2.0 tool,SignalP 4.1 tool,ProtScale tool,PSORT Prediction,SOPMA and SWISS-MODEL.[Result]L.ridibundus was closest to Rissa tridactyla in genetic relationship.The IRF7 gene was 1 242 bp in length,and encoded a hydrophilic protein IRF7 which was without signal peptide or transmembrane do-main.IRF7 had a molecular weight of about 46 ku,mainly located in the nucleus,and was predicted to be neither transmembrane protein nor secretory protein.The secondary structure of IRF7 was mainly composed of random coils,and its tertiary structure was 78.73%similar to the model protein IRF7 of Arenaria interpres.[Conclusion]The conservation of IRF7 gene is poor.关键词
红嘴鸥/干扰素调节因子7/克隆/生物信息学分析/病毒防御Key words
Larus ridibundus/interferon regulatory factor 7/clone/bioinformatics analysis/virus defense分类
畜牧业引用本文复制引用
王一涵,张宏莉,张慧,马俊蕊,项勋,段纲,常华..红嘴鸥IRF7基因克隆与生物信息学分析[J].福建农林大学学报（自然科学版）,2025,54(4):513-521,9.基金项目
云南省重大科技专项(202302AA310020、202202AE090024) （202302AA310020、202202AE090024）
云南农业大学教育教学改革研究类项目(YNAUKCSZJG2023009) （YNAUKCSZJG2023009）
云南农业大学第二批校级一流本科课程(2021YLKC017) （2021YLKC017）
云南农业大学第四批校级一流本科课程(A1010103001). （A1010103001）
