福建农林大学学报（自然科学版）2025，Vol.54Issue(4)：567-575,9.DOI:10.13323/j.cnki.j.fafu(nat.sci.).202408035
3类香菇菌株体细胞不亲和性与遗传差异
Somatic incompatibility and genetic difference of 3 types Lentinula edodes strains
摘要
Abstract
[Objective]The relationship between somatic incompatibility(SI)and genetic difference of Lentinula edodes strains was analyzed to provide a basis for evaluating the genetic differences of L.edodes strains via SI test.[Method]Dikaryon strains were con-structed with 11 monosporous strains of L.edodes,and their genetic differences were analyzed by SI reaction and molecular markers of inter-simple sequence repeat(ISSR)and sequence-related amplified polymorphism(SRAP).[Result]Fifteen dikaryon strains of 3 types were obtained with clear genetic source and SI reactions divided into compatibility and incompatibility.Among them,type Ⅰdikaryon strains were prone to demonstrate"companion antagonism",while type Ⅱ and Ⅲ were prone to show typical antagonistic reactions.Combined analysis with ISSR and SRAP molecular markers showed that the genetic similarity coefficients among the 15 strains ranged from 0.43 to 1.00,and they could be divided into 2 categories when the similarity coefficient was 0.58,which soundly reflected the genetic origin of the tested strains.[Conclusion]The nuclei differences between L.edodes dikaryotic strains affected the levels of somatic incompatibility reactions between individuals,and the results of antagonistic reactions corresponded to the genetic background differences of the strains.关键词
双核菌株/拮抗/遗传关系/分子标记/细胞质遗传Key words
dikaryon strain/antagonism/genetic relationship/molecular marker/cytoplasm inheritance分类
农业科技引用本文复制引用
罗莉媛,王车昭,周雨灵,聂梦涵,刘新锐..3类香菇菌株体细胞不亲和性与遗传差异[J].福建农林大学学报（自然科学版）,2025,54(4):567-575,9.基金项目
福州市现代菌业项目(榕财农(指)[2019]113号). （榕财农(指）
