纺织工程学报2025，Vol.3Issue(3)：26-33,8.
基于非织造布复合改良MRS液体培养基比色检测啤酒腐败菌的新方法
A new colorimetric detection method for beer spoilage lactic acid bacteria based on non-woven fabric composite and improved MRS liquid culture medium
摘要
Abstract
As beer spoilage lactic acid bacteria(LAB)can lead to beer spoilage,the detection of LAB is an inev-itable requirement for beer industry.This paper reported a new colorimetric detection method for beer spoilage LAB based on non-woven fabric composite and improved De Man,Rogosa and Sharp medium(MRS)liquid medium.The method can detect beer spoilage LAB(1 CFU/15 mL)within 48 h methyl red and bromocresol green was used as the indicator of beer spoilage LAB.If the filter membrane changes from green to yellow,it is positive for spoilage bacteria;if not,it is negative.Deionized water was replaced with 75%beer mixture in the improved MRS medium for simulating the native environment of spoilage bacteria LAB.And 50 U/mL catalase was used to activate beer spoilage bacteria in low metabolism state.Membrane filtration method was also used to enrich beer spoilage LAB from low-concentration beer.Hydrophilic non-woven fabric can adsorb liquid medi-um to provide efficient nutrition supply.The method is low in cost,high in efficiency and easy to operate to use in the factory.关键词
啤酒腐败菌/乳酸菌/高灵敏检测/非织造布复合液体培养基/比色法Key words
beer spoilage bacteria/lactic acid bacteria/high sensitive detection/non-woven composite liquid medium/colorimetric method分类
轻工纺织引用本文复制引用
刘姗,向阳,周丰,高飞,鲁振坦..基于非织造布复合改良MRS液体培养基比色检测啤酒腐败菌的新方法[J].纺织工程学报,2025,3(3):26-33,8.基金项目
国家自然科学基金资助项目(52273060) （52273060）
湖北省科技厅揭榜挂帅项目(2022BEC049). （2022BEC049）
