黑龙江动物繁殖2025，Vol.33Issue(3)：45-49,5.DOI:10.19848/j.cnki.ISSN1005-2739.2025.06.0008
DREAM基因在小鼠睾丸不同发育阶段的表达及定位
Expression and localization of DREAM gene in stages of the development of mouse testes
摘要
Abstract
To investigate the expression levels and distribution of DREAM gene and its proteins at different developmental stages of testicular tissues of mice and their possible involvement in testicular development and spermatogenesis,the expression of DREAM gene was detected through Real time PCR,and the expression and localization of DREAM protein through immunohistochemistry and Western Blot methods.The results showed that the expression of DREAM gene was detected at different developmental stages of mouse testes;DREAM protein was mainly detected in spermatocytes,stromal cells,and seminal vesicle.However,a relatively low expression in spermatozoa and round sperm cells and no expression in basal cells.It can be concluded that the expression of DREAM gene happens in all stages of mouse testicular development and spermatogenesis and is mainly localized in spermatocytes and stromal cells.关键词
小鼠/睾丸/DREAM基因/睾丸发育/精子生成Key words
mice/testicles/DREAM gene/testicular development/spermatogenesis分类
生物科学引用本文复制引用
岳续朋,杨瑞,李婷..DREAM基因在小鼠睾丸不同发育阶段的表达及定位[J].黑龙江动物繁殖,2025,33(3):45-49,5.基金项目
遵义医科大学珠海校区教育教学改革计划项目(ZH202211B、ZH202217B) （ZH202211B、ZH202217B）
2024年度贵州省科技创新人才团队项目(黔科合人才CXTD[2025]046) （黔科合人才CXTD[2025]046）
贵州省科技厅项目(黔科合基础-ZK[2022]一般619) （黔科合基础-ZK[2022]一般619）
2022年度珠海市社会发展领域科技计划项目 （）
