吉林中医药2025，Vol.45Issue(6)：711-717,7.DOI:10.13463/j.cnki.jlzyy.2025.06.021
不同频率推拿手法对神经病理性疼痛大鼠脊髓背角突触可塑性的影响
Effects of different frequencies of massage manipulations on the synaptic plasticity in the dorsal horn of the spinal cord in rats with neuropathic pain
摘要
Abstract
Objective To investigate the effects of different frequencies of massage manipulations on the expression of BDNF and SYN1 and the analgesic mechanism of synaptic plasticity in the dorsal horn of the spinal cord in rats with neuropathic pain.Methods The levels of paw withdrawal mechanical threshold(PMWT)and paw withdrawal thermal latency(PWTL)were measured before surgery and on the first,third,seventh,and fourth days after surgery.On the 14th day after surgery,the level of IL-1β was detected by ELISA,and the expression of BDNF and SYN1 in the dorsal horn of the spinal cord was detected by Western blot.TUNEL staining was used to assess the apoptosis of spinal cord neurons,and transmission electron microscopy was used to observe the ultrastructure of synapses in the dorsal horn of the spinal cord.Results On the first and third days after surgery,compared with the control group,the levels of PMWT and PWTL were all decreased in the other groups(P<0.01),and on the 7th and 14th days after operation,the levels of PMWT and PWTL of the low-,medium-and high-frequency groups were higher than those of the model group(P<0.05 or P<0.01).Compared with the model group,the IL-1β and apoptosis rates of the low-,medium-and high-frequency groups were decreased(P<0.01),with a most significant decrease in the medium-frequency group,and the level of BDNF was decreased in the low-and medium-frequency groups(P<0.01),and the expression of SYN1 was increased in the medium-frequency group(P<0.05),and the synaptic active zone became longer(P<0.01),the thickness of synaptic dense matter in the high-frequency group and the pseudo-massage group was thickened(P<0.05),while the low-and medium-frequency groups showed a significant thickening(P<0.01),and the synaptic cleft was widened in the high-frequency group(P<0.05),and the low-and medium-frequency groups and the sham massage group showed a significant widening(P<0.01),with the medium-frequency group showing the best improvement effect.Conclusion Massage has an analgesic effect,with the medium-frequency group showing the best effect,whose mechanisms involve reducing the level of BDNF,increasing the expression of SYN1,inhibiting neuronal apoptosis,promoting synaptic repair and regeneration,and changing synaptic plasticity.关键词
推拿/手法频率/神经病理性疼痛/突触可塑性Key words
massage/manipulation frequency/neuropathic pain/synaptic plasticity分类
中医学引用本文复制引用
郑玉娇,卢栋明,郑皓馨,陈玲女,梁英业,陶俊宇,杨鹏,庞军,唐宏亮..不同频率推拿手法对神经病理性疼痛大鼠脊髓背角突触可塑性的影响[J].吉林中医药,2025,45(6):711-717,7.基金项目
国家自然科学基金资助项目(82374611) （82374611）
广西自然科学基金项目(2023GXNSFDA026012) （2023GXNSFDA026012）
广西科技重大专项(桂科AA23023003) （桂科AA23023003）
