江汉大学学报(自然科学版)2025,Vol.53Issue(3):37-47,11.DOI:10.16389/j.cnki.cn42-1737/n.2025.03.005
基于GWAS的超甜玉米种子发芽率调控基因预测
Gene Prediction for Regulating Germination Rate of Super Sweet Corn Seeds Based on GWAS
摘要
Abstract
The germination rate-related characters of 100 inbred lines of super sweet corn were measured to explore the significant associated loci and candidate genes regulating the germination rate of super sweet corn seeds.Using the super sweet corn 56K gene chip,the MLM(Q+K)model was adopted to locate a total of 5 SNPs correlated with the germination rate of super sweet corn seeds through GWAS analysis,and 43 functionally annotated genes were found within the upper and lower 200 kb confidence interval.Based on literature comparison,8 candidate genes(Zm00001eb057130,Zm00001eb057170,Zm00001eb057030,Zm00001eb145440,Zm00001eb145480,Zm00001eb057150,Zm0000-1eb145520,Zm00001eb057110)were found to be related to the regulation of seed germination rate of super sweet corn.Among them,5 candidate genes played a role in the embryonic development of seeds,thus directly affecting the germination rate of seeds,and 3 candidate genes indirectly affected the germination rate of seeds,mainly by participating in the root growth and development of seeds.The results of this study can provide theoretical support for breeding super sweet corn varieties with high germination rates,which provides a reference for fine mapping and cloning of genes related to germination rate.关键词
超甜玉米/发芽率/全基因组关联分析/候选基因Key words
super sweet corn/germination rate/genome-wide association analysis/candidate genes分类
农业科技引用本文复制引用
崔妮妮,魏贺勤,余育林,余海兵,程昕昕..基于GWAS的超甜玉米种子发芽率调控基因预测[J].江汉大学学报(自然科学版),2025,53(3):37-47,11.基金项目
国家自然科学基金委员会应急管理项目(31440066) (31440066)
安徽省高校自然科学研究重大项目(KJ2019ZD57) (KJ2019ZD57)
安徽科技学院科研发展基金项目(FZ230126) (FZ230126)