口腔颌面外科杂志2025，Vol.35Issue(3)：181-189,9.DOI:10.12439/kqhm.1005-4979.2025.03.003
应激颗粒形成对人牙周膜细胞凋亡及对LPS刺激下炎症相关因子表达及ROS生成的影响
Effects of stress granule formation on apoptosis of human periodontal ligament cells and the expression of inflammatory-related factors and ROS generation under LPS stimulation
摘要
Abstract
Objective:To investigate the formation of stress granule(SG)in human periodontal ligament cells(hPDLCs)under stress,and its effect on the apoptosis,the expression of inflammatory factors,and the generation of reactive oxygen species(ROS)under lipopolysaccharide(LPS)stimulation.Methods:Sodium arsenite(SA)was used to induce SG formation in hPDLCs,and immunofluorescence staining was used to quantify SG formation.Live and dead cell staining,real-time quantitative polymerase chain reaction(RT-qPCR)and immunofluorescence staining were used to detect the localization and expression of cysteinyl aspartate-specific proteinase 3(Caspase-3)in hPDLCs to evaluate the effect of SG formation on apoptosis.RT-qPCR was used to detect the mRNA expression changes of inflammatory-related cytokines interleukin 1β(IL-1β)and interleukin 6(IL-6)during LPS stimulation.ROS detection was used to assess the effect of SG on LPS-induced ROS levels of hPDLCs.Results:SA successfully induced SG formation in hPDLCs.The results of immunofluorescence staining showed that Caspase-3 was localized in SG,which significantly inhibited the apoptosis of hPDLCs.RT-qPCR results indicated that SG could downregulate the mRNA relative expression of LPS-stimulated inflammatory cytokines in hPDLCs.ROS detection showed that SG inhibited LPS-induced ROS generation in hPDLCs.Conclusion:SG can protect hPDLCs from stress-induced apoptosis and inhibit the expression of LPS-triggered inflammatory-related factors and ROS generation.关键词
应激颗粒/凋亡/牙周炎/活性氧Key words
stress granule/apoptosis/periodontitis/reactive oxygen species分类
口腔医学引用本文复制引用
徐辉,乔广艳,苏俭生..应激颗粒形成对人牙周膜细胞凋亡及对LPS刺激下炎症相关因子表达及ROS生成的影响[J].口腔颌面外科杂志,2025,35(3):181-189,9.基金项目
国家自然科学基金(81873715、82170913) （81873715、82170913）
上海市科学技术委员会项目(201409006200) （201409006200）
