口腔颌面外科杂志2025，Vol.35Issue(3)：190-198,9.DOI:10.12439/kqhm.1005-4979.2025.03.004
梓醇/GelMA水凝胶的构建及其抗氧化性能的体外研究
In vitro study on the preparation of catalpol/GelMA hydrogel and its antioxidant properties
张春雷 1王迪 2杨景 2韩鸿洋 2杨澍 2逄博 2宋涛2
作者信息
- 1. 哈尔滨医科大学附属第一医院口腔颌面外科,哈尔滨医科大学口腔医学院,哈尔滨 150000||中俄口腔生物医学研究所,哈尔滨 150500
- 2. 哈尔滨医科大学附属第一医院口腔颌面外科,哈尔滨医科大学口腔医学院,哈尔滨 150000
- 折叠
摘要
Abstract
Objective:To investigate the effects of catalpol on rat Schwann cell(RSC)and establish a catalpol/methacrylated gelatin(GelMA)hydrogel,followed by in vitro evaluation of its biocompatibility and antioxidant properties.Methods:First,the optimal concentration of catalpol for acting on RSC96 cell(rat Schwann cell line)was determined through CCK8 assay.Subsequently,the effects of catalpol on RSC96 cell migration were analyzed by using scratch assay and Transwell assay.Real-time quantitative polymerase chain reaction(RT-qPCR)was employed to assess catalpol's influence on mRNA expression levels of glial fibrillary acidic protein(GFAP),nerve growth factor(NGF),brain-derived neurotrophic factor(BDNF)and glial cell line-derived neurotrophic factor(GDNF).An oxidative stress model was established using H2O2,and catalpol's antioxidant capacity was evaluated through CCK8 assay and reactive oxygen species(ROS)staining.Finally,the mechanical properties,biocompatibility,antioxidant capacity of catalpol/GelMA hydrogel,along with its effects on RSC96 cell proliferation,were systematically investigated.Results:The optimal concentration of catalpol in GelMA for acting on RSC96 cells was determined to be 10 µmol/L.At this concentration,catalpol significantly promoted the proliferation and migration of RSC96 cell,upregulated mRNA expression levels of GFAP,NGF,BDNF and GDNF,and attenuated oxidative stress-induced damage in RSC96 cells.The constructed catalpol/GelMA hydrogel demonstrated excellent capability for RSC96 cell encapsulation and exhibited favorable biocompatibility.Furthermore,it effectively enhanced RSC96 cell proliferation and exerted protective effects against H2O2-induced cytotoxicity.Conclusion:Catalpol/GelMA hydrogel has good in vitro cell biocompatibility and antioxidant properties.关键词
梓醇/施万细胞/氧化应激/水凝胶/体外细胞实验Key words
catalpol/Schwann cells/oxidative stress/hydrogel/in vitro cell experiments分类
口腔医学引用本文复制引用
张春雷,王迪,杨景,韩鸿洋,杨澍,逄博,宋涛..梓醇/GelMA水凝胶的构建及其抗氧化性能的体外研究[J].口腔颌面外科杂志,2025,35(3):190-198,9.
