落叶果树2025，Vol.57Issue(3)：13-17,5.DOI:10.13855/j.cnki.lygs.2025.03.003
冬樱花组培快繁技术研究
Research on tissue culture and rapid propagation technology of Cerasus cerasoides
摘要
Abstract
A rapid in vitro propagation system was successfully established for Cerasus cerasoides(D.Don)Sok.using semi-lignified stem segments as explants.The optimal surface sterilization method involved sequential treatment with 75％ethanol for 30 s and 5％sodium hypochlorite solution for 10 min.The initial culture medium was MS basal medium supplemented with 1.0 mg/L 6-benzylaminopurine(6-BA)and 0.1 mg/L indole-3-butyric acid(IB A).The optimal proliferation medium was MS containing 0.3 mg/L 6-BA and 0.2 mg/L IBA,yielding a multiplication coefficient of 4.6.Rooting was optimally induced in half-strength MS medium supplemented with 0.5 mg/L α-naphthalene acetic acid(NAA),resulting in 84％rooting rate with an average of 6.24 roots per plantlet.The survival rate of transplanting using perlite+peat soil+vermiculite(volume ratio 1∶3∶2)substrate is 92％.关键词
冬樱花/增殖培养/生根培养/移栽Key words
Cerasus cerasoides/proliferation culture/rooting cultivation/transplant分类
农业科技引用本文复制引用
徐丽,张冰,谭钺,宗晓娟,曾沛源,魏海蓉..冬樱花组培快繁技术研究[J].落叶果树,2025,57(3):13-17,5.基金项目
山东省重点研发计划资助(2024TZXD035,2024LZGCQY013) （2024TZXD035,2024LZGCQY013）
现代农业产业技术体系专项资金资助(CARS-30-1-08) （CARS-30-1-08）
国家自然科学基金面上项目(No.32172534). （No.32172534）
