落叶果树2025，Vol.57Issue(3)：18-22,5.DOI:10.13855/j.cnki.lygs.2025.03.004
铬胁迫对苹果组培苗生理特性的影响
Effect of chromium stress on the physiological characteristics of apple tissue culture seedlings
摘要
Abstract
The effects of different concentrations of potassium dichromate on the physiological characteristics of P1 tissue cultured seedlings of apple rootstock were studied.The subculture medium was treated with potassium dichromate at four concentrations of 25,50,100 and 200 mg/L,with 0 mg/L as the control.Physiological indicators were measured every 7 days after treatment.The results showed that tissue cultured seedlings were negatively affected by 25～200 mg/L chromium treatment,which was positively correlated with the treatment concentration and varied with the extension of treatment time until it decreased.The chlorophyll content in seedlings decreases with increasing treatment concentration;After the same treatment,the content began to decrease on the 21st day,and the greater the chromium treatment concentration,the greater the decrease;The activities of soluble sugars,antioxidant enzymes SOD,POD and CAT increased with the increase of chromium treatment concentration,and showed a trend of first increasing and then decreasing with the prolongation of chromium treatment time.After 7 days of treatment,low concentrations of 25 and 50 mg/L potassium dichromate had little effect on the seedlings,and the enhancement of antioxidant enzyme activity was relatively small.At 14 or 21 days of treatment,the activities of antioxidant enzymes SOD,POD and CAT in the seedlings treated with high concentrations of 100 and 200 mg/L decreased,and the chlorophyll content decreased.The seedlings were subjected to chromium stress toxicity.关键词
铬/胁迫/苹果组培苗/生理特性Key words
chromium/stress/apple tissue culture seedlings/physiological characteristics分类
农业科技引用本文复制引用
许朝茹,何东宇,陈梓雯,李莉娇,李玲,陈修德..铬胁迫对苹果组培苗生理特性的影响[J].落叶果树,2025,57(3):18-22,5.基金项目
山东省重点研发计划(重大科技创新工程)(2021LZGC021) （重大科技创新工程）
山东省果品产业技术体系-栽培与土肥岗位(SDAIT-06-04) （SDAIT-06-04）
山东省重点研发计划(竞争性创新平台)项目(2022CXPT017). （竞争性创新平台）
