林产化学与工业2025，Vol.45Issue(3)：97-106,10.DOI:10.20195/j.issn.0253-2417.2024042
不同采摘期椒蒿VOCs的变化规律及标志成分研究
Research on the Changes and Characteristic Components of VOCs in Artemisia dracunculus L.at Different Picking Periods
摘要
Abstract
To investigate the dynamic change patterns of volatile organic compounds(VOCs)in Artemisia dracunculus L.,gas chromatography-ion mobility spectrometry(GC-IMS)combined with orthogonal partial least squares discriminant analysis(OPLS-DA)was employed to detect and analyze the changes in VOCs of A.dracunculus during different harvest periods(15-90 days,S1-S6).The results indicated that a total of 63 signal peaks were identified,corresponding to 39 VOCs,including 7 ketones,9 aldehydes,9 alcohols,6 terpenes,3 esters,and 5 other substances.The peak intensity of terpenes was relatively high at S1(15 days)and S4(60 days),while the aldehydes reached the highest level at S6(90 days)and the lowest at S2(30 days).The terpene substances showed a trend of first decreasing,then increasing,and finally decreasing again.The ketone substances showed a trend of first increasing,then decreasing,and finally increasing again.The aldehyde and alcohol compounds showed a W-shaped trend throughout the picking period,while the ester substances showed an M-shaped trend.The OPLS-DA method was used to analyze the differences in VOCs of A.dracunculus at different harvest periods.The results revealed that the VOCs in S1,S2,and S3 samples were relatively similar,as were S5 and S6.A total of 20 VOCs with a variable importance in projection(VIP value)≥1.0 could be used as rapid identification markers for A.dracunculus.Comprehensive analysis indicated that the main marker components in fresh A.dracunculus leaves were(E)-2-hexenal,(E)-2-pentenal,(Z)-4-heptenal,1-pentanol,1-penten-3-ol,1-penten-3-one,1-propanethiol,2-methyl butanal,2-methylpropanoic acid,2-octanone,3-methyl-2-butenal,acetone,α-pinene,β-ocimene,β-pinene,ethyl 3-methylbutanoate,γ-terpinene,limonene,methyl 3-methylbutanoate,and myrcene.关键词
椒蒿/采摘期/气相色谱-离子迁移谱(GC-IMS)/正交偏最小二乘法判别分析(OPLS-DA)Key words
Artemisia dracunculus L./picking period/gas chromatography-ion mobility spectrometry(GC-IMS)/orthogonal partial least squares-discriminant analysis(OPLS-DA)分类
化学化工引用本文复制引用
后春静,孙艺铭,杨嘉璐,王林庆,张贝儿,郭勤..不同采摘期椒蒿VOCs的变化规律及标志成分研究[J].林产化学与工业,2025,45(3):97-106,10.基金项目
新疆维吾尔自治区自然科学基金青年项目(2023D01C221) （2023D01C221）
柔性陶瓷及全固态金属锂电池创新团队项目(2024D14005) （2024D14005）
大学生创新创业训练计划项目(202413558005) （202413558005）
