华西口腔医学杂志2025，Vol.43Issue(3)：336-345,10.DOI:10.7518/hxkq.2025.2024357
经多巴胺或硅烷化修饰的氧化石墨烯钛表面涂层的体外成骨性能研究
In vitro osteogenic performance study of graphene oxide-coated titanium surfaces modified with dopamine or silane
摘要
Abstract
Objective This study aimed to compare the osteogenic performance differences of titanium surface coat-ings modified by dopamine or silanized graphene oxide,and to provide a more suitable modification scheme for ti-tanium surface graphene oxide coatings.Methods Tita-nium was subjected to alkali-heat treatment and then modified with dopamine and silanization,respectively,followed by coating with graphene oxide.Control and ex-perimental groups were designed as follows:pure titanium(Ti)group;titanium after alkali-heat treatment(Ti-NaOH)group;titanium after alkali-heat treatment and silanization modification(Ti-APTES)group;titanium after alkali-heat treatment and dopamine modification(Ti-DOPA)group;titanium with silanization-modified surface decorated with gra-phene oxide(Ti-APTES/GO)group;titanium with dopamine-modified surface decorated with graphene oxide(Ti-DOPA/GO)group.The physical and chemical properties of the material surfaces were analyzed using scanning electron micros-copy(SEM),contact angle goniometer,X-ray photoelectron spectroscopy(XPS),and Raman spectrometer.The prolifera-tion and adhesion morphology of mouse embryonic osteoblast precursor cells MC3T3-E1 on the material surfaces were observed by cell viability detection and immunofluorescence staining followed by laser confocal microscopy.The effects on the osteogenic differentiation of MC3T3-E1 cells were studied by alkaline phosphatase(ALP)staining,alizarin red staining and quantification,and real-time quantitative polymerase chain reaction.Results After modification with gra-phene oxide coating,a thin-film-like structure was observed on the surface under SEM.The hydrophilicity of all experi-mental groups was improved,among which the Ti-DOPA/GO group had the best hydrophilicity.XPS and Raman spec-troscopy analysis showed that the modified materials exhibited typical D and G peaks,and XPS revealed the presence of a large number of oxygen-containing functional groups on the surface.CCK8 assay showed that all groups of materials had no cytotoxicity,and the proliferation level of the Ti-APTES/GO group was higher than that of the Ti-DOPA/GO group.Under the laser confocal microscope,the cells in the Ti-DOPA/GO and Ti-APTES/GO groups spread more fully.The Ti-DOPA/GO and Ti-APTES/GO groups had the deepest ALP staining,and the Ti-APTES/GO group had the most alizarin red-stained mineralized nodules and the highest quantitative result of alizarin red staining.In the Ti-DOPA/GO and Ti-APTES/GO groups,the expression of the early osteogenic-related gene RUNX2 reached a relatively high level,while in the expression of the late osteogenic-related genes OPN and OCN,the Ti-APTES/GO group performed better than the Ti-DOPA/GO group.Conclusion Ti-APTES/GO significantly outperformed Ti-DOPA/GO in promoting the adhesion,proliferation,and in vitro osteogenic differentiation of MC3T3-E1 cells.关键词
钛/氧化石墨烯/表面改性/成骨分化Key words
titanium/graphene oxide/surface modification/osteogenic differentiation分类
医药卫生引用本文复制引用
吴清霖,赖颖真,黄艳玲,谢泽宇,林彦吟..经多巴胺或硅烷化修饰的氧化石墨烯钛表面涂层的体外成骨性能研究[J].华西口腔医学杂志,2025,43(3):336-345,10.基金项目
福建省自然科学基金(2022J011408) （2022J011408）
厦门市医疗卫生指导性项目(3502Z20244ZD1134) （3502Z20244ZD1134）
厦门医学院校级科研项目(K2023-01) Fujian Natural Science Foundation Project(2022J011408) （K2023-01）
Xiamen City Medical and Health Guiding Project(3502Z20244ZD1134) （3502Z20244ZD1134）
Scientific Research Project of Xiamen Medical College(K2023-01) （K2023-01）
