林业科学研究2025，Vol.38Issue(3)：29-38,10.DOI:10.12403/j.1001-1498.20240247
紫花含笑TPS基因家族鉴定及与萜烯类物质代谢关系分析
Identification of TPS Gene Family and Its Association with Terpenoid Metabolism in Michelia crassipes
摘要
Abstract
[Objective]Terpenes are the substances with the highest number and content of volatile com-ponents in Michelia crassipes.This study aims to systematically identify TPS gene family members in M.crassipes,and elucidate their functional roles in terpenoid metabolism and floral aroma formation.[Meth-ods]Based on the transcriptome sequencing data of M.crassipes,the TPS genes family of M.crassipes was identified by bioinformatics methods.The data obtained by qRT-PCR,headspace solid-phase micro-extraction and gas chromatography-mass spectrometry were used to analyze the relationship between TPS genes expression level and terpene metabolic content in different stages.[Results]A total of 10 TPS members were identified from the transcriptome data of M.crassipes.The molecular weight of the protein encoded by the TPS genes family members ranged from 57 810.20 to 113 803.45 Da,and the number of amino acids encoded ranged from 511 to 987.The results of phylogenetic analysis showed that the TPS gene family of M.crassipes was divided into three gene subfamilies.The TPS-e/f gene subfamily members lacked Motif 1,Motif 3 and Motif 5,and the TPS-a gene subfamily members contained 6 Motifs.The TPS-b gene subfamily member C41348.3 lacked Motif 3 and the remaining members contained 6 Motifs.TPS genes C41348.6 and C41348.3 were significantly highly expressed in stage V,while C31983.0 and C40582.0 also exhibited high expression at this stage.In terms of terpene content,A-ocimene,α-Caryo-phyllene,β-Ocimene,and cadinadiene were notably increased at stage IV.The content of δ-elemene,α-Caryophyllene,β-Ocimene increased in stage V.The correlation between gene expression and terpene content was analyzed.The TPS-b gene subfamily members C41348.3 and C41348.6 were correlated with the synthesis of the 1,2-bis[(E)-prop-1-enyl]cyclobutane.C31983.0 in the TPS-e/f gene subfamily of M.crassipes was positively correlated with three types of sweet fruit aroma compounds.[Conclusion]The compounds A-ocimene,α-Caryophyllene,δ-elemene,and cadinadiene,may contribute to the fruity floral aroma characteristic of M.crassipes at stage IV,while β-elemene,α-caryophyllene,and δ-elemene are likely involved in aroma formation at stage V.TPS genes C41348.6 and C41348.3 may participate in the biosynthesis of 1,2-bis[(E)-prop-1-enyl]cyclobutane,whereas C31983.0 appears to regulate the formation of sweet fruity aroma-related compounds such as β-elemene,α-caryophyllene,and δ-elemene.Overall,the TPS gene family plays a crucial role in terpene biosynthesis.This study provides an in-depth character-ization of TPS gene family members in M.crassipes,offering a theoretical foundation for future research on the key genes involved in terpenoid biosynthesis in its petals.关键词
紫花含笑/生物信息学/TPS基因家族/表达分析/挥发性萜类物质Key words
Michelia crassipes/TPS genes family/Bioinformatics/Expression analysis/Volatile terpenoids分类
农业科技引用本文复制引用
及浩楠,肖宇光,牛小云,张志龙,刘军,姜景民,刁姝..紫花含笑TPS基因家族鉴定及与萜烯类物质代谢关系分析[J].林业科学研究,2025,38(3):29-38,10.基金项目
项目名称浙江省农业(花卉新品种选育)新品种选育重大科技专项(2021C02071-3) （花卉新品种选育）
