林业科学研究2025，Vol.38Issue(3)：39-50,12.DOI:10.12403/j.1001-1498.20240327
沙棘Dof基因家族的全基因组鉴定及表达分析
Identification and Expression Analysis of the Sea Buckthorn Dof Gene Family in the Whole Genome
摘要
Abstract
[Objective]To Identify Dof(DNA binding with one finger)gene family members in Mongolian Sea Buckthorn(Hippophae rhamnoides subsp.mongolica)and Explore Key Dof Genes regulating oil syn-thesis and accumulation in fruit flesh[Methods]Bioinformatics approaches were used to identify the Dof gene family members in the sea buckthorn genome,followed by a systematic analysis of their protein do-mains,conserved motifs,cis-regulatory elements,and phylogenetic relationships.Absolute quantification transcriptome sequencing(Unique Molecular Identifier RNA-seq,UMI RNA-seq)was performed on the fruit flesh of 12 sea buckthorn germplasm lines.Key candidate Dof genes were identified based on the expres-sion levels of Dof family members and the oil content in the fruit flesh.[Results]A total of 47 Dof members were identified in the sea buckthorn genome,unevenly distributed across 12 chromosomes.Ten motifs were identified,with cis-regulatory elements related to plant oil synthesis and development.The Dof gene family was classified into three subfamilies,and phylogenetic analysis revealed that some sea buckthorn Dofs were closely related to the Dofs regulating oil synthesis in soybean(NP001236530.2,NP001315363.1).UMI RNA-seq analysis of the fruit flesh during different developmental stages of the sea buckthorn germplasms showed that the expression of HrDof1.2(Hic_asm_12.312 8)gene during the or-ange fruit stage was significantly higher than in the green fruit stage,and was closely correlated with the variation in oil content in the corresponding germplasms.[Conclusion]Members of the sea buckthorn Dof gene family are involved in oil synthesis and development.The expression of the HrDof1.2 gene is closely related to the oil content in the fruit flesh,providing a scientific basis for further elucidation of the regulat-ory mechanism of oil synthesis and accumulation by Dof genes in sea buckthorn.关键词
沙棘/Dof/基因家族/油脂合成/表达分析Key words
sea buckthorn/dof/gene family/oil synthesis/expression analysis分类
农业科技引用本文复制引用
鹿梅,丁健,罗红梅,海鹭,张滨..沙棘Dof基因家族的全基因组鉴定及表达分析[J].林业科学研究,2025,38(3):39-50,12.基金项目
国家自然科学基金面上项目(32371913) （32371913）
辽宁省科技计划联合计划(基金)项目(2023JH2/101700242) （基金）
辽宁省"兴辽英才计划"项目(XLYC2203001) （XLYC2203001）
大连民族大学教学改革研究与实践项目(ZD202301) （ZD202301）
中央高校基本科研业务费项目(0919140114). （0919140114）
