农业生物技术学报2025，Vol.33Issue(7)：1503-1519,17.DOI:10.3969/j.issn.1674-7968.2025.07.008
蝴蝶兰WOX基因家族的鉴定、表达和互作蛋白分析
Identification,Expression and Interacting Protein Analysis of WOX Gene Family in Phalaenopsis equestris
摘要
Abstract
WOX(WUSCHEL-related homeobox)is a plant-specific transcription factor family with important regulatory roles in plant growth and stress adaptation.Phalaenopsis equestris is a widely cultivated flower and of great economic importance in orchids.The identification and analysis of WOX family members is helpful to speculate its function in P.equestris development.Ten members of PeWOX family were characterized by bioinformatics analysis in this study,which were low hydrophilic proteins and most were localized in the nucleus.Phylogenetic analysis showed that PeWOX13A and PeWOX13B belonged to ancient clade,PeWOX9,PeWOX11,and PeWOX12 belonged to intermediate clade,and others belonged to modern clade.Members of 3 clades showed obvious differences in protein structure.Expression profiling of PeWOX genes by qRT-PCR revealed their tissue-specific expression,suggesting that they might have functionally differentiation and were responsible for different organ development.Promoter analysis and the response of PeWOX genes to hormones and stresses by qRT-PCR suggested that PeWOX members were involved in the hormonal and stress response pathways.Interaction protein analysis revealed interaction relationships between PeWOX proteins and other transcription factors,epigenetic modifiers,and kinases,indicating that PeWOXs might have complex regulatory mechanisms.This study provides valuable information for further exploration of PeWOX function.关键词
蝴蝶兰/PeWOX/系统进化分析/表达谱/蛋白质互作Key words
Phalaenopsis equestris/PeWOX/Phylogenetic analysis/Expression profile/Protein interaction分类
农业科技引用本文复制引用
张婷,石子豪,郑甜甜,于薪,徐艳,易庆平,朱德艳..蝴蝶兰WOX基因家族的鉴定、表达和互作蛋白分析[J].农业生物技术学报,2025,33(7):1503-1519,17.基金项目
特色花卉生物育种湖北省工程研究中心开放课题重点项目(2022ZD007 （）
2023ZD002) （）
湖北省中央引导地方科技发展专项(2022BGE262) （2022BGE262）
