农业生物技术学报2025，Vol.33Issue(7)：1520-1530,11.DOI:10.3969/j.issn.1674-7968.2025.07.009
热应激加剧双酚A致荷斯坦牛睾丸支持细胞铁死亡的发生
Heat Stress Aggravates Ferroptosis of Holstein cattle(Bos taurus)Sertoli Cells Induced by BPA
摘要
Abstract
Bisphenol A(BPA),an environmental estrogen analogue,is capable of inducing perturbations within the antioxidant system of sertoli cells(SCs),thereby giving rise to oxidative stress.Concurrently,SCs which serve as the pivotal cells responsible for nourishing and supporting spermatogenic cells,exhibit a high susceptibility to alterations in temperature.To explore whether heat stress exacerbates the occurrence of BPA-induced ferroptosis in SCs in Holstein cattle(Bos taurus),bovine testis SCs were divided into blank control group(Con),BPA treatment group(BPA),heat stress group(HS),BPA and heat stress treatment group(HS+BPA)in this study.Western blot and RT-qPCR methods were used to detect the proliferation and secretion factors of bovine testis SCs and the genes and proteins related to ferroptosis,the content of reactive oxygen species(ROS)and free iron and mitochondrial membrane potential was detected by fluorescent probe method,and the change of malondialdehyde(MDA)content was detected by colorimetric method.The results showed compared with the control group,HS+BPA group reduced the expression of glial cell-derived neurotrophic factor(GDNF),bone morphogenetic protein 4(BMP4),C-X-C motif chemokine ligand 12(CXCL12)and proliferating cell nuclear antigen(PCNA),androgen-binding protein(ABP)related to proliferation and secretion of SCs.The expression of ferroptosis-related genes and proteins ferritin heavy chain 1(FTH1),solute carrier family 7 member 11(SLC7A11),glutathione peroxidase 4(GPX4)were extremely significantly decreased(P＜0.01).Meanwhile,the expression levels of prostaglandin-endoperoxide synthase 2(PTGS2),acyl-coA synthetase long-chain family member 4(ACSL4)and transferrin receptor(TFRC)in SCs of testis were extremely significantly increased(P＜0.01)and the difference was significant compared with BPA group and HS group(P＜0.05).Compared with the control group,the content of ROS,MDA and free iron in testicular SCs in the co-treatment group increased,while the content of GSH and the relative value of JC-1 staining decreased,and the difference was significant compared with the BPA group and HS group(P＜0.05).These results indicated that 43℃ for 1 h increased the incidence of ferroptosis and decreased the expression of cell proliferation and secretory factors in Holstein bovine testis SCs after heat stress with 160 μmol/L BPA pretreated.This study provides research directions and fundamental materials for addressing the issue of reduced fertility in dairy cows during summer production.关键词
荷斯坦牛/睾丸支持细胞(SCs)/热应激/双酚A/铁死亡Key words
Holstein cattle/Sertoli cells(SCs)/Heat stress/Bisphenol A(BPA)/Ferroptosis分类
畜牧业引用本文复制引用
马旭辉,戴光明,李华涛,刘锡武,曹雷..热应激加剧双酚A致荷斯坦牛睾丸支持细胞铁死亡的发生[J].农业生物技术学报,2025,33(7):1520-1530,11.基金项目
山东省自然科学基金(ZR2023MC027) （ZR2023MC027）
青岛市惠民科技计划(23-1-3-7-zyyd-nsh) （23-1-3-7-zyyd-nsh）
