农业生物技术学报2025，Vol.33Issue(7)：1531-1542,12.DOI:10.3969/j.issn.1674-7968.2025.07.010
番茄红素对H2O2诱导的猪卵巢颗粒细胞氧化损伤的保护作用
Protective Effect of Lycopene Against H2O2-induced Oxidative Damage in Porcine Ovarian Granulosa Cells
摘要
Abstract
The ovaries of sows(Sus scrofa)suffer oxidative stress can affect their reproductive performances.Lycopene(LYC),a natural carotenoid,has strong antioxidant properties and effectively alleviate oxidative damage in the body.The aim of this work was to investigate the protective effect of LYC against hydrogen peroxide(H2O2)-induced apoptosis and oxidative damage in porcine ovarian granulosa cells(PGCs).In this study,healthy and sexually mature porcine ovarian tissues were collected to isolate and culture PGCs,dividing the experiment into 3 groups:The control group,the H2O2 model group,and the LYC+H2O2 treatment group.Cell counting kit-8(CCK-8)was used to detect cell viability to screen the optimal concentration of H2O2 damage and LYC treatment,and the kits were used to detect ROS and related antioxidant indexes.qRT-PCR was used to detect the mRNA expression levels of the genes related to cell apoptosis B cell lymphoma-2(BCL-2),BCL-2-associated X protein(BAX),cysteinyl aspartate-specific proteinase-3(CASP3),cell proliferation proliferating cell nuclear antigen(PCNA),insulin-like growth factor 2(IGF2),antioxidant enzyme system catalase(CAT),manganese superoxide dismutase(MnSOD),copper-zinc superoxide dismutase(CuZnSOD),and steroid hormone biosynthesis steroidogenic acute regulatory protein(STAR),3β-hydroxysteroid dehydrogenase 1(HSD3B1),cytochrome P450 11A1(CYP11A1),cytochrome P450arom(CYP19A1).Results indicated that 5 μmol/L LYC was most effective in promoting PGC viability,while 200 μmol/L H2O2 represented the IC50 for PGCs.Compared to the control group,the H2O2 model group showed extremely significantly elevated intracellular ROS fluorescence,ROS levels,and malondialdehyde(MDA)content(P＜0.01),alongside decreased levels of total superoxide dismutase(T-SOD),total antioxidant capacity(T-AOC),glutathione peroxidase(GSH-Px),and catalase(CAT)(P＜0.05).The mRNA expression levels of BCL-2,PCNA,IGF2,CuZnSOD,MnSOD,CAT,STAR,HSD3B1,CYP11A1 and CYP19A1 were extremely significantly downregulated in the H 2 O 2 model group(P＜0.01),while BAX,CASP3 were extremely significantly upregulated(P＜0.01)compared with the control group.In the LYC+H2O2 group,intracellular ROS fluorescence,ROS levels,and MDA content extremely significantly decreased compared to the H2O2 model group(P＜0.01),whereas cell viability,T-SOD,T-AOC,GSH-Px,and CAT levels extremely significantly increased(P＜0.01).Additionally,LYC treatment significantly upregulated the mRNA expression of PCNA,IGF2,BCL-2,CuZnSOD,MnSOD,CAT,STAR,CYP11A1,and CYP19A1(P＜0.05)and significantly downregulated BAX and CASP3(P＜0.05)compared with the H2O2 model group.These results suggested that LYC had a protective effect on porcine ovarian granulosa cells by alleviating oxidative stress damage caused by H2O2.This study provides theoretical basis for the application of LYC in the swine industry.关键词
番茄红素(LYC)/氧化应激/猪卵巢颗粒细胞(PGCs)/细胞凋亡/繁殖性能Key words
Lycopene(LYC)/Oxidative stress/Porcine ovarian granulosa cells(PGCs)/Apoptosis/Reproductive performances分类
畜牧业引用本文复制引用
杨振清,胡光玲,石庆武,李昌红,陈广,王杰,张依裕,敖政..番茄红素对H2O2诱导的猪卵巢颗粒细胞氧化损伤的保护作用[J].农业生物技术学报,2025,33(7):1531-1542,12.基金项目
国家自然科学基金(32460838) （32460838）
促进与加拿大、澳大利亚、新西兰及拉美地区科研合作与高层次人才培养项目(留金美[2021]109号) （留金美[2021]109号）
贵州省科技计划项目(黔科合基础-ZK[2021]一般166、黔科合支撑[2021]一般150) （黔科合基础-ZK[2021]一般166、黔科合支撑[2021]一般150）
国家重点研发计划子课题(2022YFD1100308-01) （2022YFD1100308-01）
