军事医学2025，Vol.49Issue(5)：370-373,4.DOI:10.7644/j.issn.1674-9960.2025.05.007
顶空气相色谱法测定肟类EN螯合剂中残留溶剂
Determination of residual solvents in amidoxime EN chelating agents by headspace gas chromatography
朱金寿 1肖炳坤 2缪潇瑶 2杨芳 3黄荣清2
作者信息
- 1. 青海大学药学系,西宁 810016||军事科学院军事医学研究院,北京 100850
- 2. 军事科学院军事医学研究院,北京 100850
- 3. 青海大学药学系,西宁 810016
- 折叠
摘要
Abstract
Objective To establish an analytical method for the determination of methanol and ethanol solvent residues in amidoxime EN chelating agents by headspace gas chromatography.Methods The samples were analyzed in a GC-2030 gas chromatograph system using a Shimadzu HS-20 fully automated headspace injector and quantified by an internal standard method.The samples were separated and detected by the DB-WAX capillary column with a flame ionization detector(FID).The thermostatic furnace temperature was 70 ℃ and the equilibrium time of the sample bottle was 30 min.The sample flow path temperature was 150℃ and the carrier gas was high pure nitrogen.As temperature programming,the initial column temperature of 40 ℃ lasted 9 min and increased to 200 ℃ at 40 ℃/min for 2 min.Results The peak areas of the residual solvents in EN chelator showed good linearity(R2＞0.999)in the mass concentration range investigated,the detection limits of solvent methanol and ethanol were 0.25 μg/mL and 0.30 μg/mL respectively,and the limits of quantification were 1.25 μg/mL and 1.50 μg/mL,respectively.The recovery of residual solvent addition ranged from 97.10％to 102.23％,and the RSD value was less than 5.00％.Conclusion This method is sensitive enough to be used for the determination of solvent residues in amidoxime EN chelating agents.关键词
顶空气相色谱法/全自动顶空进样器/内标法/肟类EN螯合剂/甲醇/乙醇Key words
headspace gas chromatography/automatic headspace sampler/internal standard method/amidoxime EN chelating agent/methanol/ethanol分类
化学引用本文复制引用
朱金寿,肖炳坤,缪潇瑶,杨芳,黄荣清..顶空气相色谱法测定肟类EN螯合剂中残留溶剂[J].军事医学,2025,49(5):370-373,4.
