吉林中医药2025，Vol.45Issue(6)：621-626,6.DOI:10.13463/j.cnki.jlzyy.2025.06.001
志不安于肾不寐模型大鼠听觉区脑组织凋亡因子表达研究
Study on the apoptosis expression of the brain tissue of the auditory region in rats with insomnia caused by the kidney failing to store the will
摘要
Abstract
Objective To investigate the pathogenesis of insomnia by detecting the protein expression levels of Bcl-2,Bax,Caspase-3 and NF-κB in the brain tissue of the auditory region and the kidney tissue of rats with insomnia caused by the kidney failing to store the will.Methods A total of 16 SPF male SD rats were randomly divided into a control group(n=8)and a model group(n=8).In the model group,D-galactose was injected subcutaneously in the back of the rat neck and p-chlorophenylalanine was injected intraperitoneally to prepare a model of rats with insomnia caused by the kidney failing to store the will,while the control group was injected with the same dose of normal saline.HE staining was used to observe the pathological changes in renal tissue of the two groups,and immunohistochemistry(IHC)and WB were used to detect the expression levels of Bcl-2,Bax,Caspase-3 and NF-κB in the relevant organs of the two rat groups.Results Compared with the control group,the terminal body weight was significantly reduced in the model group(P<0.001),and the sleep latency was significantly prolonged,yet the sleep time was significantly shortened(P<0.001),while the residence time in the target quadrant was increased,the number of times to enter the target quadrant was increased,and the movement distance in the target quadrant was increased,and the target platform was found to have the longest trajectory(P<0.05).HE staining of the kidney in the model group showed disorders in the glomeruli and glomerular structure,and the IHC results showed that the expression of Bcl-2 in brain tissue in the auditory area and the kidney tissue of the model group was decreased,and the ratio of Bcl-2/Bax was decreased(P<0.05),while the expression levels of Bax,Caspase-3 and NF-κB were increased(P<0.05).The WB results showed that the expression of Bcl-2 in the kidney tissue and brain tissue of the auditory area of the model group was decreased(P<0.05),while the expression levels of NF-κB,Caspase-3 and Bax were increased significantly(P<0.05).Conclusion The changes in the level of apoptosis in the brain tissue and kidney tissue are correlated with the occurrence of insomnia caused by the kidney failing to store the will.关键词
中医不寐五神分型/不寐/志不安于肾/凋亡Key words
TCM classification of insomnia based on the five emotions/insomnia/kidney failing to store will/apoptosis分类
医药卫生引用本文复制引用
王慧,张星平,陈旭,吴金鸿,贾宏林,闫德祺,梁政亭..志不安于肾不寐模型大鼠听觉区脑组织凋亡因子表达研究[J].吉林中医药,2025,45(6):621-626,6.基金项目
国家自然科学基金项目(82160873,81960837,81560762,81260526) （82160873,81960837,81560762,81260526）
新疆维吾尔自治区自然科学基金重点项目(2022D01D48) （2022D01D48）
全国名老中医药专家传承工作室(国中医药人教函[2022]75号) （国中医药人教函[2022]75号）
国家中医药管理局"十四五"高水平中医药重点学科项目(国中医药人教函[2023]85号) （国中医药人教函[2023]85号）
新疆"十四五"中医学重点学科项目(新教函[2022]112号) （新教函[2022]112号）
