农业生物技术学报2025，Vol.33Issue(7)：1568-1579,12.DOI:10.3969/j.issn.1674-7968.2025.07.013
大黄鱼atic基因的分子特性及其表达量与肌苷酸含量的相关性分析
Molecular Characterizations of atic Gene and the Correlation Analysis Between Its Expression Levels and Inosine Monophosphate Content in Large Yellow Croaker(Larimichthys crocea)
摘要
Abstract
The declination of flesh quality has become a major concern in the cultivation of large yellow croaker(Larimichthys crocea).For investigating the critical roles of aminoimidazole carboxamide ribonucleotide transformylase/inosine monophosphate cyclohydrolase(ATIC)in inosine monophosphate(IMP)formation,the relevant CDS of Lcatic was cloned and characterized.The CDS of Lcatic was 1 776 bp and encoded a polypeptide of 591 amino acids,for which the molecular weight was estimated to be 64.08 kD,with 1 N-glycosylation site and 56 phosphorylation sites.The deduced protein,LcATIC,possesses methylglyoxal synthetase(MGS)and aminoimidazole carboxamide ribonucleotide formyltransferase(AICARFT))/IMP cyclohydrolase(IMPCHase)structural domains.The genomic DNA sequence of Lcatic is subdivided into 15 exons and 14 introns,exhibiting a genome organization similar to that of atic in other teleost fish.Phylogenetic analysis of the amino acid sequence revealed that LcATIC aligned most with the Collichthys lucidus ATIC,sharing the similarity of 99.5％,they gathered in one branch and then joined the teleost branch.Lcatic mRNA was found to be expressed at a high level in the liver tissue,expressed at intermediate levels in gill,brain,intestine,and muscle tissue,and expressed at a low level in the stomach tissue.With the increase of body weight,Lcatic expression in muscle tissues of L.crocea showed a gradually increasing trend,which was consistent with the trend of gradually increasing IMP content in these muscle tissues.The Spearman correlation analysis further showed that the Lcatic mRNA expression was positively correlated with IMP content in the muscles of different-sized L.crocea(P＜0.05,rs=0.931).These results suggest the critical role of Lcatic in IMP synthesis and provide basis reference for the study of the regulation mechanism of Lcatic on flesh quality of large yellow croaker.关键词
大黄鱼/肌苷酸(IMP)/氨基咪唑氨甲酰核苷酸转甲酰基酶/次黄嘌呤核苷酸环水解酶(ATIC)/分子特征Key words
Larimichthys crocea/Inosine monophosphate(IMP)/Aminoimidazole carboxamide ribonucleotide transformylase/inosine monophosphate cyclohydrolase(ATIC)/Molecular characterization分类
水产学引用本文复制引用
何亮银,张雅婷,尤钰鑫,曾智军,孙赵韩,史晓丽..大黄鱼atic基因的分子特性及其表达量与肌苷酸含量的相关性分析[J].农业生物技术学报,2025,33(7):1568-1579,12.基金项目
福建省自然科学基金(2023J011070) （2023J011070）
宁德师范学院科研项目(2022FZ10) （2022FZ10）
