农业生物技术学报2025，Vol.33Issue(7)：1589-1597,9.DOI:10.3969/j.issn.1674-7968.2025.07.015
注射IAG重组质粒对克氏原螯虾IAG、mhc、gasp的表达及第二性征的影响
Effects of IAG Recombinant Plasmid Injection on the Expression of IAG,mhc,gasp and Secondary Sexual Characteristics of Procambarus clarkii
摘要
Abstract
The insulin-like androgenic gland hormone(IAG)gene plays an important role in sexual differentiation and growth in decapod crustaceans.To investigate the effects of recombinant plasmids containing IAG on the expression of endogenous genes(IAG)and growth-related genes myosin heavy chain(mhc)and growth arrest-specific protein(gasp)as well as secondary sexual characteristics in Procambarus clarkii.This study involved injecting different doses(0.5,1.0 and 3.0 μg/g)of IAG recombinant plasmids into the muscle of healthy minor female P.clarkii weighing between 6.5～16.5 g.The weight,abdominal development,and expression of genes IAG,mhc,and gasp in the tissues of the experimental shrimp were monitored at 1,7,14,and 28 d,respectively.The results showed that compared with the control group,the copy number of plasmids in the experimental group was positively correlated with the concentration of injection.The copy number in each tissue showed a distribution pattern of muscle＞gonads＞appendages＞hepatopancreas.The degree of plasmid digestion was the highest between 3 and 7 d,and at 28 d,the plasmid was almost completely digested.After 1 d of injection,the expression of mhc in muscles significantly increased at injection concentrations of 1.0 and 3.0 μg/g(P＜0.05),and body weight gain was also significantly higher than that of the control group(P＜0.05).Within 28 d after injection,female crayfish with an injection concentration of 3.0 μg/g had the fastest ovarian development,while the control group had the slowest development.No masculinization was observed in the abdomen of all female shrimps.Before the mass digestion of plasmids,the expression levels of IAG in the gonads and gasp in the muscles of the experimental group were lower than those in the control group,and their expression was inhibited.The results showed that the injection of IAG recombinant plasmid had certain effects on ovarian development and expression of IAG,mhc,gasp.This study provides reference for research on IAG function and gender control.关键词
克氏原螯虾/胰岛素样促雄腺激素(IAG)/重组质粒/肌球蛋白重链(mhc)/生长抑制特异性蛋白(gasp)Key words
Procambarus clarkii/Insulin-like androgenic gland hormone(IAG)/Recombinant plasmid/Myosin heavy chain(mhc)/Growth arrest-specific protein(gasp)分类
水产学引用本文复制引用
陈毅君,解颖琳,赵彤,应可,李艳和..注射IAG重组质粒对克氏原螯虾IAG、mhc、gasp的表达及第二性征的影响[J].农业生物技术学报,2025,33(7):1589-1597,9.基金项目
中央高校基本科研业务费专项资金资助项目(2662025FW008) （2662025FW008）
华中农业大学大学生科技创新基金(2022305) （2022305）
湖北省第四批现代农业产业技术体系水产产业技术体系(2023HBSTX4-05) （2023HBSTX4-05）
