农业生物技术学报2025，Vol.33Issue(7)：1598-1610,13.DOI:10.3969/j.issn.1674-7968.2025.07.016
马铃薯疮痂病菌加利利链霉菌拮抗细菌的筛选与鉴定
Screening and Identification of the Antagonistic Bacteria Against Potato Scab Streptomyces galilaeus
摘要
Abstract
Antagonistic endophytic bacteria play an important role in the biological control of potato scab disease.Using healthy tubers of disease resistant potato(Solanum tuberosum)varieties('Yunshu 304','Zhongshu 43','Zhongshu 566')as test materials and Streptomyces galilaeus as target bacteria,antagonistic bacteria were screened and identified using methods such as plate confrontation,Oxford cup method,strain morphology,physiological and biochemical characteristics,and bacterial 16S rRNA gene and gyrB gene sequencing.Their growth characteristics were analyzed and environmental adaptability was evaluated.The effectiveness of antagonistic bacteria in preventing potato scab disease was verified through pot experiments,in order to obtain efficient biocontrol strains for potato scab disease prevention and control.The results indicated that 3 strains YS304,ZS43 and ZS567 with significant antagonistic effect against pathogenic bacteria were obtained,The average diameters of bacteriostatic zones of primary and secondary screening were 18.71 and 31.57 mm,Gram staining of the strains was positive,It was identified as Bacillus velezensis combined with physiological and biochemical characteristics and 16S rRNA,gryB gene sequence analysis.The 3 strains still maintained certain antibacterial activity under pH 3～13 and 40～80℃,It was insensitive to common bactericides such as pentachloronitrobenzene,enoylmorpholine,mancozeb and haloperidine.In pot experiment of potato scab,the relative control effects were 60.84％,69.15％and 75.76％respectively.The strains YS304,ZS43 and ZS567 have good biological control effects,strong environmental adaptability and stability,they can be used as a candidate strain to control potato scab.关键词
马铃薯疮痂病/加利利链霉菌/贝莱斯芽胞杆菌/鉴定Key words
Potato scab/Streptomyces galilaeus/Bacillus velezensis/Identification分类
植物保护学引用本文复制引用
杨鑫,樊吴静,李丽淑,唐秀桦,冯志文..马铃薯疮痂病菌加利利链霉菌拮抗细菌的筛选与鉴定[J].农业生物技术学报,2025,33(7):1598-1610,13.基金项目
国家现代农业产业技术体系(CARS-09-ES19) （CARS-09-ES19）
广西农业科学院基本科研业务项目(桂农科2023YM77 （）
桂农科2021YT058) （）
