临床骨科杂志2025，Vol.28Issue(3)：444-448,5.DOI:10.3969/j.issn.1008-0287.2025.03.047
牛膝甾酮在促进大鼠骨髓间充质干细胞成骨分化中的作用
Role of inokosterone in promoting osteogenic differentiation of bone marrow mesenchymal stem cells
摘要
Abstract
Objective To investigate the role of inokosteroneon in promoting the osteogenic differentiation of bone marrow mesenchymal stem cells(BMSCs).Methods BMSCs were managed with varying doses of inokosterone.Os-teogenic differentiation was assessed by alizarin red staining,while cell proliferation activity was measured using CCK-8 assay.Cytokine expression levels were determined by ELISA,and protein expression was analyzed via Western blot.The effects of BMP2 siRNA interference and overexpression on cell proliferation were evaluated through CCK-8 assay and Western blot analysis.Results Alizarin red staining demonstrated that inokosterone significantly enhanced osteogenic differentiation of BMSCs,with particularly pronounced effects at the high dose(200 mg/L).Compared with the blank control group,all tested doses of inokosterone increased BMSC proliferation activity(P<0.05),and promoted the expression of osteogenic markers including Collagen I,ALP,OC and related differentiation proteins(P<0.05).BMP2 siRNA+inokosterone group showed significantly higher proliferation activity than the BMP2 siR-NA-only group(P<0.01).Conclusions Inokosterone promotes proliferation and osteogenic differentiation of rat BMSCs,potentially through modulation of the BMP2/Smad signaling pathway by targeting BMP2.关键词
牛膝甾酮/骨髓间充质干细胞/成骨分化/骨形态发生蛋白Key words
achyranosterone/bone marrow mesenchymal stem cells/osteogenic differentiation/bone morphogenetic proteins分类
医药卫生引用本文复制引用
孙祥燚,李敏,龙如超,杨志华,陈强,冯纯智..牛膝甾酮在促进大鼠骨髓间充质干细胞成骨分化中的作用[J].临床骨科杂志,2025,28(3):444-448,5.基金项目
浙江省杭州市科技局科技计划项目(编号:20201231Y120) （编号:20201231Y120）
