热带作物学报2025，Vol.46Issue(7)：1754-1766,13.DOI:10.3969/j.issn.1000-2561.2025.07.022
热带雨林5株生防红曲霉菌株的分离、鉴定和活性物质研究
Isolation,Identification and Active Substances Analysis of Five Biocontrol Strains of Monascus sp.from Tropical Rainforest
摘要
Abstract
Monascus is usually used in food and medical industries.To explore the biocontrol potential of Monascus and its secondary metabolites against plant biocontrol pathogens,in this study,through the isolation and identification of the fungi from humus samples in the rain forest,a total of five strains growing well on the potato dextrose agar medium were screened,the colonies were red,with typical Monascus morphological characteristics.The strains were identified as Monascus sanguineus based on the sequencing and analysis of ITS,LSU and pksKS gene sequences,The results of antagonistic experiments showed that the five strains of Monascus had better and more obvious inhibitory effects on Fusarium oxysporum f.sp.cubense,F.oxysporum f.sp.tracheiphilium,Phytophthora capsici,Colletotrichum gloeosporioides.After fermentation of Monascus with different media,high performance liquid chromatography(HPLC)and nuclear magnetic resonance analysis were carried out.It was found that Monascus could produce abundant second-ary metabolites with strong antibacterial activity on solid medium,especially on wheat bran medium.The further pot experiment proved that Monascus could effectively reduce the wilt index of cowpea,and the best biocontrol effect was obtained when the spore concentration was 106 CFU/mL.In summary,Monascus isolated from tropical rain forest showed good results of better biocontrol of plant diseases,and the secondary metabolites obtained by fermentation in rice medium had strong antibacterial activity,and could effectively control cowpea blight.Monascus strains isolated from tropical rain forest have great potential in plant disease biocontrol application,which can provide more abundant strains selection for plant disease biocontrol.关键词
红曲霉/次生代谢产物/植物病原菌/生物防治Key words
Monascus sp./secondary metabolite/phytopathogen/biological control分类
农业科技引用本文复制引用
王凌萱,都燚,孙艺函,杨扬,杨腊英..热带雨林5株生防红曲霉菌株的分离、鉴定和活性物质研究[J].热带作物学报,2025,46(7):1754-1766,13.基金项目
国家重点研发计划项目(No.2024YFD1400100) （No.2024YFD1400100）
江西省重点研发计划项目(No.20223BBF61015) （No.20223BBF61015）
农业农村部履职类开放式项目(No.JDZX-2024). （No.JDZX-2024）
