热带农业科学2025，Vol.45Issue(5)：1-8,8.DOI:10.12008/j.issn.1009-2196.2025.05.001
不同地域益智品质差异化相关基因相对表达量分析
Analysis of Gene Expression Related to the Differentiation of Alpinia oxyphylla(Miq.)Quality in Different Regions
摘要
Abstract
Terpenes and flavonoids are the main pharmacological components of the medicinal plant Alpinia oxyphylla(Miq.).Our previous studies identified several genes related to the biosynthesis of its representative components,nootkatone and kaempferol.To further identify the candidate genes responsible for the significant differences in the contents of these two substances between different regions,this study quantitatively analyzed the expression of these genes via real-time quantita-tive PCR.We compared the relative expression differences in A.oxyphylla samples from different regions and selected genes whose expression levels were consistent with the contents of nootkatone and kaempferol as key candidate genes.The results revealed that the genes Cluster-27812.24310 and Cluster-27812.43619,which are involved in the biosynthesis of nootkatone and kaempferol in A.oxyphylla,respectively,exhibited significant expression differences across different regions.In particular,the expression level of Cluster-27812.24310 was consistent with the content of nootkatone,indicating that it is a positive regulatory gene for nootkatone biosynthesis.These findings provide a reference for the functional analysis of genes related to the quality differences in A.oxyphylla and further research on the molecular mechanisms involved.关键词
益智/圆柚酮/山奈酚/候选基因/荧光实时定量法Key words
Alpinia oxyphylla(Miq.)/nootkatone/kaempferol/candidate gene/real-time quantitative PCR分类
农业科技引用本文复制引用
刘嘉褀,贾玉平,瞿云苹,罗凡,潘坤..不同地域益智品质差异化相关基因相对表达量分析[J].热带农业科学,2025,45(5):1-8,8.基金项目
国家自然科学基金(No.81660629) （No.81660629）
海南省科技专项资助重点研发计划项目(No.ZDYF2022XDNY170) （No.ZDYF2022XDNY170）
海南医学院大学生创新创业训练计划项目(No.X202111810038). （No.X202111810038）
