热带农业科学2025，Vol.45Issue(5)：23-30,8.DOI:10.12008/j.issn.1009-2196.2025.05.004
链霉菌(F2)对芒果炭疽病菌(Colletotrichum gloeosporioides)的抑菌机理研究
Antifungal Mechanism of Streptomyces F2 against Mango Anthracnose Pathogen(Colletotrichum gloeosporioides)
摘要
Abstract
Postharvest anthracnose caused by Colletotrichum gloeosporioides is a major constraint in the development of the mango industry.Our previous studies revealed that Streptomyces F2 strongly antagonizes C.gloeosporioides.To better utilize this strain,we investigated its antagonistic mechanism against C.gloeosporioides.The results showed that the F2 fermentation broth damaged the plasma membrane integrity of the spores,thereby reducing the spore germination rate.It also disrupts hyphal cell integrity,causing leakage of intracellular soluble proteins and total sugars and inhibiting hyphal growth.After 6 h of treatment,the fermentation broth induced temporary increases in the hyphal respiration rate,Na+K+-ATPase and Ca2+Mg2+-ATPase activities,and ATP concentration,possibly as a stress response.However,after 12 h of treatment,the respi-ration rates and activities of four enzymes(Na+K+-ATPase,Ca2+Mg2+-ATPase,SDH,and MDH)continuously decreased,and the ATP content decreased,significantly inhibiting hyphal physiological metabolism.The 10×diluted fermentation broth showed 98.35%control efficacy against postharvest mango anthracnose,demonstrating good biocontrol potential.The anti-fungal effect is achieved through disruption of hyphal and spore membrane integrity,coupled with inhibition of energy metab-olism and hyphal growth in C.gloeosporioides.Streptomyces F2 shows promising potential for use in biological control ap-plications.关键词
生物防治/链霉菌/胶孢炭疽病菌/抑菌机理/发酵液/芒果Key words
biocontrol/Streptomyces/C.gloeosporioides/antifungal mechanism/fermentation broth/mango分类
农业科技引用本文复制引用
陈帅,胡美姣,李敏,赵德庆,赵超..链霉菌(F2)对芒果炭疽病菌(Colletotrichum gloeosporioides)的抑菌机理研究[J].热带农业科学,2025,45(5):23-30,8.基金项目
海南省自然科学基金(No.321MS0763). （No.321MS0763）
