山东农业大学学报（自然科学版）2025，Vol.56Issue(3)：462-468,7.DOI:10.3969/j.issn.1000-2324.2025.03.010
壳聚糖盐酸盐/磺丁基-β-环糊精纳米载药体系增强CCCH-ZAP抗ALV-J活性研究
Enhancement of anti-ALV-J activities by CCCH-ZAP loaded with Chitosan Hydrochloride/Sulfobutyl-β-Cyclodextrin Nanocarrier
摘要
Abstract
CCCH-zinc-finger antiviral protein can significantly inhibit J avian leukemia virus infection and relieve the immune suppression caused by J avian leukemia virus infection.However,the application of protein drugs is limited by poor immunogenicity,stress response caused by injection,and low oral bioavailability.To explore the rational oral administration of CCCH-zinc-finger antiviral protein,chitosan hydrochloride and hydrochloride/sulfobutyl-β-cyclodextrin with good water solubility were selected and a system of ion-crosslinked chitosan hydrochloride/sulfobutyl-β-cyclodextrin was constructed to achieve the loading of CCCH-zinc-finger antiviral protein.Besides,the anti J avian leukemia virus infection virus activity,cytotoxicity,and transmembrane behavior were studied.Results showed that the system has good cell permeability,and CCCH-zinc-finger antiviral protein can be loaded successfully,with a loading rate of 61.5%at pH=7.4.After loading,the inhibition rate of CCCH-zinc-finger antiviral protein against J avian leukemia virus infection was significantly enhanced,with an inhibition rate of 55.0%at 72 hours,and the difference of inhibitory activity enlarged with time.The study indicates that chitosan hydrochloride/sulfobutyl-β-cyclodextrin system is a promising carrier for protein drugs,providing a basis for oral delivery of protein drugs in the prevention of avian leukemia virus.关键词
壳聚糖/药物负载/锌指抗病毒蛋白/抗病毒活性Key words
Chitosan/drug loading/CCCH-ZAP/antiviral activity分类
化学引用本文复制引用
易春荣,李敏,付孟悦,吕文燕,李映..壳聚糖盐酸盐/磺丁基-β-环糊精纳米载药体系增强CCCH-ZAP抗ALV-J活性研究[J].山东农业大学学报（自然科学版）,2025,56(3):462-468,7.基金项目
山东省自然科学基金面上基金(ZR2023MB094) （ZR2023MB094）
