山东农业大学学报（自然科学版）2025，Vol.56Issue(3)：505-516,12.DOI:10.3969/j.issn.1000-2324.2025.03.015
不同基质配方对黑木耳胞外酶活、农艺性状及产量的影响
Different Matrix Formulations on Extracellular Enzyme Activity,Agronomic Traits,and Yield of Auricularia auricula
摘要
Abstract
The cultivation substrate affects the extracellular enzyme activity,agronomic traits,and yield of Auricularia auricula during its growth and development.To investigate the effects of different substrate formulations on these parameters,common wood species from Guizhou—Fagus longipetiolata,Broussonetia papyrifera,Bothrocaryum controversum,and Betula luminifera—are selected and combined into 15 formulations for cultivating A.auricula.The activities of lignin degrading enzyme system,cellulose degrading enzyme system and hemicellulose degrading enzyme system are determined at different mycelial development stages,along with the agronomic traits and yield of fruiting bodies,following by correlation(P<0.05)and principal component analysis of each indicator.A comprehensive evaluation of each formulation is conducted using the membership function method.The results show that different formulations affect the extracellular enzyme activity.Additionally,the activity within the same enzyme system exhibit significant positive correlations across different growth stages and among different enzymes.Furthermore,multiple agronomic traits also show significant positive correlations.Significant differences are observed in agronomic traits and yield among different formulations:ear length(66.99～101.72 mm),ear width(36.28～98.95 mm),ear thickness(1.34～1.96 mm),elasticity(0.75～3.00 Pa),rehydration rate(5.10～14.90%),fresh yield per bag(47.00～189.00)g),dry yield per bag(5.00-24.00 g),and biological efficiency(31.33-126.00%).Finally,through comprehensive analysis using the membership function method,formulations 2,4,8,9,10,11,12,13,and 14 demonstrate superiority over the control.Considering the cultivation costs,the yield and the output value of A.auricula,formulation 9 is ultimately selected as the most cost-effective option.关键词
黑木耳/配方/酶活/农艺性状/产量Key words
Auricularia auricula/formulation/enzyme activity/agronomic traits/yield分类
园艺学与植物营养学引用本文复制引用
江苏燕,田风华,徐彦军,李伟,吴安波,于飞,郎云龙,牟海林,蔡兴玉,王仁贤,陈孟谈,向准..不同基质配方对黑木耳胞外酶活、农艺性状及产量的影响[J].山东农业大学学报（自然科学版）,2025,56(3):505-516,12.基金项目
贵州食用菌与茶产业循环经济技术成果转化(黔科合中引地[2024]026) （黔科合中引地[2024]026）
贵州省教育厅高等学校设施蔬菜工程研究中心平台建设项目(黔教技[2022]040号) （黔教技[2022]040号）
贵州省科技厅科技重大专项(黔科合重大专项字[2019]3005) （黔科合重大专项字[2019]3005）
贵州省教育厅自然科学研究项目(黔教合KY字[2021]054) （黔教合KY字[2021]054）
贵州省农业农村厅食用菌产业项目(黔财农2019[143]号)和贵州省教育厅农业重大产业攻关项目(黔教合KY字[2019]017) （黔财农2019[143]号）
