曲阜师范大学学报（自然科学版）2025，Vol.51Issue(3)：99-106,8.DOI:10.3969/j.issn.1001-5337.2025.3.099
前星覆葬甲线粒体全基因组序列测定和分析
Sequencing and analysis of the complete mitochondrial genome of Nicrophorus maculifrons(Coleoptera:Silphidae)
摘要
Abstract
Nicrophorus maculifrons belongs to Nicrophorinae,Silphidae,Coleoptera.In this study,high-throughput sequencing technology was used to assemble the complete mitochondrial genome sequence of N.maculifrons.The study analyzed the sequence structure,base composition,codon usage frequency,and the secondary structure of tRNA genes of N.maculifrons.At the same time,two phylogenetic trees were constructed using Bayesian and maximum likelihood methods based on the sequences of 13 protein-coding genes of the N.maculifrons and seven other species of Silphidae.The results showed that the mito-chondrial genome of N.maculifrons is 16 692 bp,including 13 protein-coding genes,22 tRNAs,2 rRNAs and a control region.The genome exhibits a significant AT bias,with an A+T content of 76.42％.Among the 22 tRNA genes,all but four,which have partial structural deficiencies,exhibit the typical clo-verleaf secondary structure.The results of two phylogenetic analyses based on 13 protein-coding genes showed that the phylogenetic relationship between the N.maculifrons and Nicrophorus nepalensis is the closest,which was consistent with the traditional morphological classification results.关键词
葬甲科/覆葬甲亚科/前星覆葬甲/线粒体基因组/系统发育Key words
Silphidae/Nicrophorinae/Nicrophorus maculifrons/mitochondrial genome/phylogeny分类
生物科学引用本文复制引用
房新民,陈超,白明,马志红,赵国庆,丁强,于莹莹,路园园,李银鸽,高雪雅,陈新..前星覆葬甲线粒体全基因组序列测定和分析[J].曲阜师范大学学报（自然科学版）,2025,51(3):99-106,8.基金项目
国家重点研发计划(2022YFC2601200) （2022YFC2601200）
国家科技基础资源调查专项(2019FY100400,2022FY100500). （2019FY100400,2022FY100500）
