农业生物技术学报2025，Vol.33Issue(7)：1440-1450,11.DOI:10.3969/j.issn.1674-7968.2025.07.003
甘薯GA20氧化酶基因的鉴定、表达及功能验证
Identification,Expression and Functional Validation of the GA20-oxidase Gene in Sweet Potato(Ipomoea batatas)
摘要
Abstract
GA20-oxidase,a key enzyme in the gibberellins(GAs)biosynthesis pathway,has been widely studied in major food crops such as rice(Oryza sativa),wheat(Triticum aestivum),and corn(Zea mays),however,research on it in sweetpotato(Ipomoea batatas)remains completely unexplored.In this study,a GA20 oxidase gene named I.batatas gibberellin 20 oxidase 1(IbGA20ox1)was identified using the full-length transcriptome and genome data of sweet potato(I.batatas),and its bioinformatics and expression pattern were analyzed,and its function was verified by overexpression in Arabidopsis thaliana.The results showed that the CDS of IbGA20ox1 gene was 1 083 bp,encoding 361 amino acids,and the molecular weight of the protein was 40.7 kD,which was an acidic hydrophile protein.Comparative analysis of protein sequences showed that GA20 oxidase in plants was highly conserved.Expression pattern analysis showed that IbGA20ox1 gene was highly expressed in sweet potato flowers,fibrous roots,leaves and buds.In leaves,this gene can be induced by gibberellin.IbGA20ox1 in A.thaliana overexpressed plants showed elongated plants,early flowering,and increased the expression of key genes of GA metabolic pathway.The results of this study provide a reference for further elucidating the functional characteristics of GA20 oxidase gene in sweet potato and revealing its molecular mechanism involved in regulating the growth and development of sweet potato.关键词
甘薯/GA20氧化酶/基因克隆/功能验证Key words
Sweet potato/GA20 oxidase/Gene cloning/Functional verification分类
农业科技引用本文复制引用
胡志芳,郭一博,赵志鹏,曾希,何境强,朱宏波..甘薯GA20氧化酶基因的鉴定、表达及功能验证[J].农业生物技术学报,2025,33(7):1440-1450,11.基金项目
国家自然科学基金-广东省联合基金(U1701234) （U1701234）
