热带作物学报2025，Vol.46Issue(7)：1554-1561,8.DOI:10.3969/j.issn.1000-2561.2025.07.003
抗菌肽MaSAMP的生信分析、基因克隆及遗传转化
Bioinformatics Analysis,Gene Cloning and Genetic Transformation of Antimicrobial Peptide MaSAMP
摘要
Abstract
An antimicrobial peptide(SAMP)derived from Microcitrus australasica can not only reduce the concentra-tion of Candidatus Liberibacter asiaticus(CLas)in the infected trees and reduce the symptoms of the diseased trees,but also induce innate immunity and prevent the infection of Huanglongbing in healthy trees that are not infected with Huanglongbing.MaSAMP was analyzed by functional structure,subcellular localization and phylogeny.The results showed that the open reading frame(ORF)of MaSAMP was 201 bp in length,encoding 67 amino acids.The molecular weight of the corresponding protein was 7.59959 kDa,the theoretical isoelectric point was 5.38,and the instability in-dex(Instability index(Ⅱ))was 33.22.The aliphatic index was 87.16％,and the grand average of hydropathicity was-0.09,indicating that the protein was a hydrophilic one.The protein contained one Dabb domain and was predominantly structured by α-helices and random coils.In terms of evolution,MaSAMP was phylogenetically close to the stress-response A/B barrel domain-containing protein CISIN_1g033887mg from Citrus sinensis and the A/B barrel do-main protein HS1 from Citrus×clementina,with protein similarity of 97.01％and 91.04％,respectively.Subcellular localization results indicated that MaSAMP was localized in chloroplasts.Furthermore,through homologous recombi-nation,a pCAMBIA1300-35S::MaSAMP-EGFP overexpression vector fused with an EGFP fluorescent tag was suc-cessfully constructed,and transgenic citrus plants were obtained using agrobacterium-mediated in planta transformation technology.This study would provide a material basis for subsequent research on the biological functions of MaSAMP.关键词
抗菌肽/澳指檬/生物信息学分析/载体构建/转基因Key words
antimicrobial peptides/Microcitrus australasica/bioinformatics analysis/vector construction/transgene分类
农业科技引用本文复制引用
廖名静,李向阳,吕远达,姜波,李娟,钟云,王婷,郑蓝艳..抗菌肽MaSAMP的生信分析、基因克隆及遗传转化[J].热带作物学报,2025,46(7):1554-1561,8.基金项目
国家外专局个人类项目(No.Y20240127) （No.Y20240127）
"十四五"广东省农业科技创新九大主攻方向"揭榜挂帅"项目(No.2024KJ29) （No.2024KJ29）
2024年度广东乡村振兴战略专项-广东省级现代农业产业技术体系创新团队建设项目(No.2024CXTD10). （No.2024CXTD10）
