农业生物技术学报2025，Vol.33Issue(7)：1476-1489,14.DOI:10.3969/j.issn.1674-7968.2025.07.006
毛竹HD-Zip转录因子Pehox14基因的克隆及其在干旱和盐胁迫下的功能分析
Cloning of HD-Zip Transcription Factor Pehox14 Gene in Moso Bamboo(Phyllostachys edulis)and Its Functional Analysis Under Drought and Salt Stress
摘要
Abstract
The homeodomain-leucine zipper(HD-Zip)protein is a specific transcription factor in plants,playing important regulatory roles in plant growth,development and environmental stress response.Moso bamboo(Phyllostachys edulis)grows rapidly and exhibits strong adaptability.It can cope with environmental stress and maintain normal growth and development under abiotic stress.In order to explore the expression pattern of the HD-Zip gene in moso bamboo under drought and salt stress,this study obtained the HD-Zip family member Pehox14(GenBank No.PQ806999)gene from moso bamboo.Sequence analysis showed that the ORF length of the Pehox14 gene was 699 bp,encoding 232 amino acids,including 3 exons and 2 introns.The Pehox14 protein had a conserved Homeodomain domain and a special HALZ(Leucine Zipper)domain.Subcellular localization showed that Pehox14 was located in nucleus.By using qRT-PCR to analyze the expression patterns under drought and salt stress conditions,the results showed that the expression of Pehox14 gene was significantly upregulated under drought and salt stress.Transgenic Arabidopsis plants were generated by Agrobacterium infection.Analysis of abiotic stress indicated that when transgenic Arabidopsis plants were subjected to mannitol and NaCl treatment,their germination rate,survival rate and plant height were significantly higher than those of control,indicating that overexpression of Pehox14 could enhance the tolerance of transgenic Arabidopsis to drought and salt stress.In addition,under stress conditions of 200 mmol/L mannitol and 200 mmol/L NaCl,the expression of stress-related genes,such as AtRD29A(responsive to desiccation 29A),AtRD22(responsive to desiccation 22),AtLEA(late embryogenesis abundant),AtSOS1(salt overly sensitive 1),AtNHX1(Na+/H+exchanger 1)and AtHKT(high-affinity K+transporter)was significantly upregulated in transgenic plants.Overall,Pehox14 plays a positive regulatory role in response to drought and salt stress.This study provides a theoretical basis for investigating the molecular regulation mechanism of HD-Zip transcription factors in stress resistance of moso bamboo.关键词
毛竹/HD-Zip转录因子/Pehox14/干旱胁迫/盐胁迫Key words
Phyllostachys edulis/HD-Zip transcription factor/Pehox14/Drought stress/Salt stress分类
农业科技引用本文复制引用
刘育佼,闫小玲,畅欣,肖雅倩,曹珊,郭小勤..毛竹HD-Zip转录因子Pehox14基因的克隆及其在干旱和盐胁迫下的功能分析[J].农业生物技术学报,2025,33(7):1476-1489,14.基金项目
国家自然科学基金(32171880) （32171880）
浙江省自然科学基金重点项目(LZ21C160001) （LZ21C160001）
