热带作物学报2025，Vol.46Issue(7)：1562-1570,9.DOI:10.3969/j.issn.1000-2561.2025.07.004
辣椒果实SGR基因的克隆与功能分析
Cloning and Functional Analysis of the SGR Gene in Capsicum annum Fruits
摘要
Abstract
STAY-GREEN(SGR)is a key gene that regulates chlorophyll degradation in plants and plays an important role in controlling plant senescence.To investigate the regulatory role of the SGR gene in the fruit coloration process of pepper,the homologous gene cloning method was employed to amplify the CaSGR gene from pepper fruit cDNA using PCR technology in this study.Bioinformatics analysis was conducted on its protein sequence,and a virus-induced gene silencing(VIGS)vector was constructed to preliminarily verify the gene function.The results showed that ORF of CaSGR was 792 bp,encoding 263 amino acids.Bioinformatics analysis revealed that SGR was relatively conserved among Solanaceae crops,and SGR had a closer evolutionary relationship with SGR from tomato and potato.CaSGR VIGS vector was constructed and injected into pepper leaves,successfully obtaining silenced plants.The silenced plants exhibited leaf whitening,fruit color changed,and CaSGR expression levels reduced significantly.Additionally,the chlorophyll and β-carotene content in the fruits of the silenced plants increased markedly.This study would provide a foundation for further elucidating the molecular regulatory mechanisms of CaSGR in pepper fruit coloration and offer theoretical support for breeding new pepper varieties with high carotenoid content.关键词
辣椒/CaSGR基因/VIGS技术/叶绿素/β-胡萝卜素Key words
Capsicum annum/CaSGR gene/VIGS technology/chlorophyll/β-carotene分类
农业科技引用本文复制引用
秦于玲,刘维侠,曹振木,刘琳,朱丹,殷晓敏,刘子记..辣椒果实SGR基因的克隆与功能分析[J].热带作物学报,2025,46(7):1562-1570,9.基金项目
海南省科技计划三亚崖州湾科技城联合项目(No.320LH067) （No.320LH067）
中央级公益性科研院所基本科研业务费专项(No.1630032022009). （No.1630032022009）
