农业生物技术学报2025，Vol.33Issue(7)：1490-1502,13.DOI:10.3969/j.issn.1674-7968.2025.07.007
垂枝桦GRF基因家族鉴定、表达及互作分析
Identification,Expression and Interaction Analysis of the GRF Gene Family in Betula pendula
摘要
Abstract
Growth-regulating factors(GRFs)are plant-specific transcription factors that play an important role in regulating plant growth and development.Based on the genome sequence of Betula pendula,in this study,analyses on GRF gene family were conducted,including their identification,gene sequence characteristics,evolutionary relationships,cis-acting elements within promoters,expression patterns and protein interaction.The results revealed that there were 10 GRF genes in the genome of B.pendula,distributed across 6 chromosomes and belonged to 6 subgroups(Ⅰ～Ⅵ).The members within the same subgroup had similar gene structures and conserved motifs,and they were all regulated by miR396.Cis-acting element analysis showed that numerous regulatory elements related to growth,hormone response,and abiotic stress were located in the promoter regions of BpeGRF genes.Expression analysis indicated that most BpeGRFs genes had significantly higher expression levels in young tissues such as apical buds compared to mature tissues.Genes like BpeGRF1,BpeGRF3 and BpeGRF4 exhibited specific high expression in apical buds.Meanwhile,the expression of most BpeGRF genes showed an initial increase followed by a decrease under gibberellin(GA)treatment.Protein interaction prediction analysis showed that widespread interactions among 8 BpeGRFs with GRF-interacting factor(GIF)BpeGIF1 and BpeGIF3,and further bimolecular fluorescence complementation(BiFC)experiments confirmed a strong interaction between BpeGRF4 and BpeGIF1.This study lays an important foundation for further exploration of the functions of GRF genes in B.pendula.关键词
垂枝桦/生长调节因子(GRF)基因家族/表达分析/蛋白互作Key words
Betula pendula/Growth-regulating factor(GRF)gene family/Expression analysis/Protein interaction分类
农业科技引用本文复制引用
王文新,江梅,葛徐汝,王小雅,黄华宏,林二培..垂枝桦GRF基因家族鉴定、表达及互作分析[J].农业生物技术学报,2025,33(7):1490-1502,13.基金项目
浙江省农业新品种选育项目(2021C02070-1) （2021C02070-1）
国家自然科学基金(32371832) （32371832）
