热带作物学报2025，Vol.46Issue(7)：1571-1583,13.DOI:10.3969/j.issn.1000-2561.2025.07.005
海南本地抗病小米辣响应象耳豆根结线虫侵染的miRNA鉴定及其靶基因预测
Identification and Target Gene Prediction of miRNA Responding to the Infection of Meloidogyne enterolobii in Local Resistant Xiaomila Pepper of Hainan,China
摘要
Abstract
Meloidogyne enterolobii is a pathogen causing significant damages to pepper production.Utilization of re-sistant cultivars is the most economical and environmentally friendly method to control this disease.A deeper under-standing of the regulatory mechanisms of resistance to root-knot nematodes could facilitate the breeding process of dis-ease-resistant cultivars.In previous studies,analysis of transcriptomic data of Hainan local M.enterolobii-resistant pepper germplasm CF25 before and after inoculation were carried out to explore metabolic pathways closely related to nematode resistance.In order to further explore the regulatory mechanisms of resistance,sRNA libraries of CF25 before and after inoculation with M.enterolobii were sequenced to identify a total of 133 differentially expressed miRNAs.Based on the criteria of P≤0.05 and|log2(FC)|≥3,33 significantly differentially expressed miRNAs were identified,including 19 known miRNAs and 14 newly discovered miRNAs.Target gene prediction for the 33 miRNAs yielded a total of 373 target genes,with miR5658-z having the highest number of predicted target genes.GO and KEGG analyses showed that the target genes were mainly enriched in pathways related to plant-pathogen interactions and signal trans-duction.It is speculated that miRNA-mediated signal transduction and plant defense responses might be important rea-sons for the resistance of CF25 to M.enterolobii.To preliminarily verify the regulatory relationships between differen-tially expressed miRNA and the target genes,six differentially expressed miRNA and the target genes were selected for qRT-PCR validation.The results showed that the differential expression trends of miRNA and target genes were con-sistent with the high-throughput sequencing results and generally conformed to the negative regulation pattern between miRNA and the target genes.The results of this study suggest that miRNA may play significant roles in the defense re-sponse to root-knot nematodes and lay the foundation for furtherly elucidating the mechanisms of miRNA-mediated nematode resistance in pepper.关键词
灌木辣椒/抗象耳豆根结线虫/sRNA测序/miRNA/靶基因Key words
Capsicum frutescens/Meloidogyne enterolobii resistance/sRNA sequencing/miRNA/target gene分类
农业科技引用本文复制引用
高畅,张志远,刘子记,陈梅红,朱婕,曹振木..海南本地抗病小米辣响应象耳豆根结线虫侵染的miRNA鉴定及其靶基因预测[J].热带作物学报,2025,46(7):1571-1583,13.基金项目
海南省自然科学基金项目(No.323RC412). （No.323RC412）
