热带作物学报2025，Vol.46Issue(7)：1594-1607,14.DOI:10.3969/j.issn.1000-2561.2025.07.007
基于转录组分析小白菜对强光条件的响应
Transcrip tome-based Analysis of Bok Choy(Brassica campestris ssp.chinensis)Response to High Light Conditions
摘要
Abstract
Bok choy(Brassica campestris ssp.chinensis)is an important vegetable crop native to China and is also one of the leafy vegetables favored by people in Hainan.The high temperature and intense sunlight in Hainan's summer greatly affect the normal growth and development of bok choy,reducing its yield and quality.At present,research on the growth of bok choy under abiotic stress is mostly focused on heat tolerance,while study on the response of bok choy to strong light stress is relatively scarce.To investigate the molecular regulatory mechanisms of bok choy in response to strong light,this study used the variety'Aijiao Huang'as the research object.We compared and analyzed the physio-logical indicators,photosynthetic characteristics,and expression levels of response genes in the leaves of bok choy un-der normal light[300 μmol/(m2·s)]and strong light[1500 μmol/(m2·s)].Under strong light,dry weight of bok choy sig-nificantly increased,and the R/S ratio gradually increased from day 0 to day 10,followed by a significant decrease on day 15.The chlorophyll content significantly decreased in the later stages.Pn,Tr,and Gs significantly increased on day 5 under strong light,and then gradually decreased,while Ci remained relatively stable.Using RNA-seq technology for transcriptome sequencing,a total of 2324 differentially expressed genes(DEGs)were identified.GO and KEGG en-richment analyses revealed 14 DEGs related to photosynthesis and photoprotection mechanisms,and 6 DEGs associated with antioxidant enzyme activity.8 DEGs were selected for qRT-PCR validation,which were consistent with the tran-scriptome sequencing results,confirming the reliability of the transcriptome analysis.Strong light had a certain impact on the antioxidant enzyme activity of bok choy.During days 0-5,the activity of SOD,POD,and CAT was induced to increase,while from days 5-15,the activity of SOD and POD gradually decreased,and CAT showed an upward trend.In summary,bok choy may rapidly respond to photosynthesis-related mechanisms and regulate antioxidant enzyme activity under strong light conditions,thereby alleviating the inhibitory effects of strong light on growth and enhancing the seedlings adaptability to strong light.关键词
小白菜/强光处理/生理响应/转录组分析/基因挖掘Key words
bok choy/strong light/physiological response/transcriptome analysis/gene mining分类
农业科技引用本文复制引用
赵颖,张厂,王旭..基于转录组分析小白菜对强光条件的响应[J].热带作物学报,2025,46(7):1594-1607,14.基金项目
海南省重点研发计划项目(No.ZDYF2022SHFZ061) （No.ZDYF2022SHFZ061）
海南省自然科学基金高层次人才项目(No.321RC1032). （No.321RC1032）
