热带作物学报2025，Vol.46Issue(7)：1608-1617,10.DOI:10.3969/j.issn.1000-2561.2025.07.008
橡胶树优良无性系品种云研73-477不同发育阶段体细胞幼胚成熟培养效率比较
Comparison of Somatic Embryo Maturation Efficiency at Different Developmental Stages of Rubber Tree Variety Yunyan 73-477
摘要
Abstract
The study investigated the effects of different exogenous hormones on somatic embryo maturation to improve the maturation efficiency of somatic embryos and increase the yield of normal mature embryos and embryo-derived plantlets in the rubber tree variety Yunyan 73-477.Primary somatic embryos from anthers at different developmental stages were cultured on media supplemented with various types and concentrations of exogenous hormones.The matu-ration rates,normal mature embryo rates,and plantlet conversion rates were comprehensively analyzed to compare maturation efficiencies and optimize hormone combinations for different developmental stages.The maturation effi-ciency increased with embryo developmental progression,with cotyledon embryos showing optimal results.The matu-ration rates exceeded 50％[range:50.74％-74.83％,mean:(66.25±6.98)％].Most mature embryos displayed bipolar structure and could normally germinate into complete plantlets,with normal mature embryo rates ranging from 35.0％-59.96％[mean:(47.80±6.89)％]and plantlet conversion rates ranging from 15.62％-49.17％[mean:(26.67±8.99)％].Torpedo-shaped embryos showed intermediate performance,while heart-shaped embryos performed the poorest,both with maturation rates below 31％[means:(30.05±12.68)％and(29.33±10.93)％,respectively].Most mature embryos from the stages were malformed(leaf-like or callus-like)and failed to develop into complete plantlets.The normal ma-ture embryo rates were only(15.64±7.01)％and(5.46±4.28)％,respectively,32.16％and 42.34％lower than cotyledon em-bryos.Plantlet conversion rates were(7.22±3.41)％and(0.83±0.80)％,merely 0.27-fold and 0.03-fold of cotyledon embryos.Optimal exogenous hormones varied with developmental stages:heart-shaped embryos required 6-BA 0.5 mg/L+NAA 0.15 mg/L+KT 0.25 mg/L,torpedo-shaped embryos needed ABA 0.4 mg/L+GA3 0.5 mg/L+6-BA 2.0 mg/L+NAA 0.15 mg/L,and cotyledon embryos performed best with ABA 0.1 mg/L+GA3 1.0 mg/L+6-BA 1.5 mg/L+NAA 0.2 mg/L.关键词
橡胶树/成熟培养/体细胞幼胚/正常成熟体胚/体胚植株Key words
Hevea brasiliensis/maturation culture/somatic embryos/normal mature embryos/embryo-derived plantlets分类
农业科技引用本文复制引用
桂明春,于静娟,方芳,罗荣惠,缪佳,李玲,梁国平,孙小龙,柳觐..橡胶树优良无性系品种云研73-477不同发育阶段体细胞幼胚成熟培养效率比较[J].热带作物学报,2025,46(7):1608-1617,10.基金项目
云南省农业联合专项(No.202401BD070001-127) （No.202401BD070001-127）
云南省科技人才与平台计划重点实验室项目(No.202405AG340001) （No.202405AG340001）
云南省基础研究计划重点项目(No.202401AS070083). （No.202401AS070083）
