肿瘤预防与治疗2025，Vol.38Issue(6)：449-456,8.DOI:10.3969/j.issn.1674-0904.2025.06.001
构建放射感应性LuxR/LuxI放大环路调控p53高表达及肿瘤靶向增敏作用
Constructing a Radiation-Induced LuxR/LuxI Amplification Loop to Reg-ulate p53 Overexpression and Enhance Tumor Targeting Sensitivity
摘要
Abstract
Objective:To construct a radiation-induced positive feedback loop cArGs-LuxR/luxI and utilize the X-ray spatiotemporal effect to obtain targeting tumors with sustained high expression of p53,achieving radiotherapy sensitization.Methods:Using the retroviral vector Lvool as the backbone,reporter gene vectors(pE6-EGFP,pLuxI-luxR-EGFP,pE6-LuxI-luxR-EGFP)and p53 expression vector(pE6-p53,pLuxI-luxR-p53,pE6-LuxI-luxR-p53)were constructed.Transfect H1299 and A549 cell lines separately,administer different doses of X-ray irradiation,and observe the intensity of EGFP expression.Western blotting was used to detect p53 expres-sion.Flow cytometry was used to detect cell apoptosis,while cell cloning experiments,comet electrophoresis,and other ex-periments were used to detect the radiosensitizing effect of radiotherapy.Observe tumor inhibition effect in nude mouse tumor experiments,and immunohistochemical detection of p53 expression in tumor and adjacent tissues.Results:After sequencing verification,the vectors described above were successfully constructed.The pE6-LuxI-LuxR-p53 vector exhibits radiosensitivi-ty and maintains sustained high expression of p53,with the strongest expression observed at 4 Gy.The radiotherapy sensitiza-tion ratio is 1.38.In transplanted tumors,the pE6-LuxI-luxR-p53+IR treatment group showed the strongest anti-tumor effect(P＜0.01).The expression level of p53 increased in irradiated wild tumor tissues(P＜0.05),while there was no p53 expres-sion in the adjacent tissues of the wild tumor.Conclusion:Under irradiation induction,the pE6-LuxI-LuxR-p53 vector tar-gets the high expression of p53 and has significant radiation sensitization and tumor inhibition effects.关键词
P53动力学/正反馈环路/放疗增敏/抑癌作用Key words
P53 kinetics/Positive feedback loop/Radiosensitization/Anticancer effect分类
医药卫生引用本文复制引用
王梅,吴川,吴自飞,高明权,王卫东..构建放射感应性LuxR/LuxI放大环路调控p53高表达及肿瘤靶向增敏作用[J].肿瘤预防与治疗,2025,38(6):449-456,8.基金项目
This study was supported by grants from Science and Technology Department of Sichuan Province(No.2022NSFSC0051). 四川省自然科学基金(编号:2022NSFSC0051) （No.2022NSFSC0051）
