肿瘤预防与治疗2025，Vol.38Issue(6)：457-463,7.DOI:10.3969/j.issn.1674-0904.2025.06.002
基于转录组测序分析全反式维甲酸对HBL-100细胞的影响
Investigating the Effects of All-Trans Retinoic Acid on HBL-100 Cells Using RNA-Seq Technology
摘要
Abstract
Objective:This study aims to explore the impact of all-trans retinoic acid(ATRA)on gene expression in the breast epithelial cell line HBL-100,with the goal of elucidating the potential mechanisms of ATRA underlying breast physiol-ogy and pathology.Methods:Gene expression changes in HBL-100 cells before and after ATRA treatment were analyzed u-sing RNA-Seq technology.Differentially expressed genes(DEGs)were identified with the criteria of|log2FC|≥1 and P＜0.05.The DAVID online tool was employed for Gene Ontology(GO)enrichment analysis and KEGG pathway analysis to de-termine the biological functions and signaling pathways of the DEGs.Additionally,the STRING database was used to construct a protein-protein interaction(PPI)network,and key hub genes were identified using the cytoHubba plugin.Results:A total of 580 DEGs were identified,with 303 up-regulated and 277 downregulated.Upregulated genes were mainly involved in processes such as viral defense,immune response,and apoptosis,while downregulated genes were associated with hormone signaling,cell adhesion,and metabolic pathways.KEGG analysis of upregulated genes showed enrichment in immune signaling pathways,including NOD,TOLL,and RIG-I signaling pathways,while downregulated genes were significantly enriched in pathways related to endoplasmic retic-ulum protein processing,PI3K-Akt signaling pathway,and metabolic processes.PPI network analysis identified 10 upregu-lated hub genes,including STAT1,CXCL10,and DDX58,as well as 10 downregulated hub genes,such as SPP1,APOE,and CANX,which may play crucial regulatory roles under ATRA treatment.Conclusion:This study demonstrates that ATRA significantly affects the gene expression profile of HBL-100 cells,particularly genes related to immune response and cell sig-naling.These findings provide new insights into the role of ATRA in breast epithelial cells and lay the groundwork for further exploration of ATRA's potential in breast cancer prevention and treatment.关键词
全反式维甲酸/转录组测序技术/乳腺上皮细胞/生物信息学/差异表达基因Key words
All-trans retinoic acid/RNA-Seq technology/Breast epithelial cells/Bioinformatics/Differentially expressed genes分类
生物学引用本文复制引用
蔡玉莹,马佳艺,王晓茹,孙睿,殷继明,王珊珊..基于转录组测序分析全反式维甲酸对HBL-100细胞的影响[J].肿瘤预防与治疗,2025,38(6):457-463,7.基金项目
This study was supported by grants from Sino-German Center for Research Promotion(No.M-0200). 中德科学中心[中国国家自然科学基金委员会(NSFC)和德国科学基金会(DFG)]中德合作交流项目(编号:M-0200) （No.M-0200）
